Strict paternal transmission of mitochondrial DNA of Chlamydomonas species is explained by selection against maternal nucleoids
Strict paternal transmission of mitochondrial DNA of Chlamydomonas species is explained by selection against maternal nucleoids
复制标题
衣藻物种线粒体 DNA 的严格父系传递是通过针对母系核素的选择来解释的
作者：
S. Nakamura;H. Aoyama;R. van Woesik
细胞器DNA的非孟德尔遗传在大多数植物和动物中很常见。在这里，我们研究了涉及线粒体DNA转移的遗传机制。我们先后回交（F5）两个可育株的单细胞同花受精的单倍体藻类莱茵衣藻和史密斯衣藻，以匹配核背景和研究传输模式的线粒体DNA的PCR分析的cob基因序列。线粒体DNA是严格的父系遗传。为了研究亲本线粒体DNA的行为，我们使用F5子代形成合子并分离单个合子。结果表明，母体线粒体类核的选择性消失发生在受精卵形成后3 - 6 h。
Summary. The non-Mendelian inheritance of organelle DNA is common in most plants and animals. Here we examined inheritance mechanisms involved in the transfer of mitochondrial DNA. We successively backcrossed (to F5) two interfertile strains of the unicellular isogamous haploid algae Chlamydomonas reinhardtii and Chlamydomonas smithii to match nuclear backgrounds and examine transmission patterns of mitochondrial DNA by PCR analysis of cob gene sequences. Mitochondrial DNA was strictly transmitted paternally. To investigate the behavior of parental mitochondrial DNA, we used F5 progeny to form zygotes and isolated single zygotes. The results showed selective disappearance of maternal mitochondrial nucleoids occurred between 3 and 6 h after zygote formation.