Listeria floridensis sp nov., Listeria aquatica sp nov., Listeria cornellensis sp nov., Listeria riparia sp nov and Listeria grandensis sp nov., from agricultural and natural environments
Listeria floridensis sp nov., Listeria aquatica sp nov., Listeria cornellensis sp nov., Listeria riparia sp nov and Listeria grandensis sp nov., from agricultural and natural environments
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DOI：
10.1099/ijs.0.052720-0
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发表时间：
2014-06-01
影响因子：
2.8
通讯作者：
Wiedmann, Martin
中科院分区：
文献类型：
--
作者：
den Bakker, Henk C.;Warchocki, Steven;Wiedmann, Martin
对美国两个州（科罗拉多州和佛罗里达州）的农业和自然环境进行采样，得到了18株类似李斯特菌的分离株，使用传统方法无法将它们归类到先前描述的物种中。通过全基因组测序和传统表型方法，我们鉴定出了5个新物种，每个新物种与目前已描述的物种相比，全基因组平均BLAST核苷酸同一性（ANIb）均低于85%。基于16S rRNA基因序列和31个保守基因座的氨基酸序列进行的系统发育分析表明，李斯特菌属内存在四个得到充分支持的分支：（i）一个分支代表单核细胞增生李斯特菌、马尔氏李斯特菌、无害李斯特菌、威尔斯李斯特菌、西尔利格森李斯特菌和伊氏李斯特菌，我们将其称为狭义李斯特菌；（ii）一个分支由弗莱施曼李斯特菌和两个新描述的物种组成，即水生李斯特菌新种（模式菌株FSL S10 - 1188(T)=DSM 26686(T)=LMG 28120(T)=BEI NR - 42633(T)）和佛罗里达李斯特菌新种（模式菌株FSL S10 - 1187(T)=DSM 26687(T)=LMG 28121(T)=BEI NR - 42632(T)）；（iii）一个分支由罗库尔特李斯特菌、魏恩斯泰凡李斯特菌和三个新物种组成，即康奈尔李斯特菌新种（模式菌株TTU A1 - 0210(T)=FSL F6 - 0969(T)=DSM 26689(T)=LMG 28123(T)=BEI NR - 42630(T)）、格兰德李斯特菌新种（模式菌株TTU A1 - 0212(T)=FSL F6 - 0971(T)=DSM 26688(T)=LMG 28122(T)=BEI NR - 42631(T)）和河岸李斯特菌新种（模式菌株FSL S10 - 1204(T)=DSM 26685(T)=LMG 28119(T)=BEI NR - 42634(T)）；（iv）一个包含格雷李斯特菌的分支。基因组和表型数据表明这些新物种无致病性。
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T)=DSM 26686(T)=LMG 28120(T)=BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T)=DSM 26687(T)=LMG 28121(T)=BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T)=FSL F6-0969(T)=DSM 26689(T)=LMG 28123(T)=BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T)=FSL F6-0971(T)=DSM 26688(T)=LMG 28122(T)=BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T)=DSM 26685(T)=LMG 28119(T)=BEI NR-42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic.