Human Fast Skeletal Myosin Light Chain 2 cDNA: Isolation, Tissue Specific Expression of the Single Copy Gene, Comparative Sequence Analysis of Isoforms and Evolutionary Relationships
Human Fast Skeletal Myosin Light Chain 2 cDNA: Isolation, Tissue Specific Expression of the Single Copy Gene, Comparative Sequence Analysis of Isoforms and Evolutionary Relationships
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DOI：
10.1080/1042517031000154952
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发表时间：
2003-10
期刊：
影响因子：
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通讯作者：
S. Sachdev;M. Raychowdhury;S. Sarkar
中科院分区：
文献类型：
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作者：
S. Sachdev;M. Raychowdhury;S. Sarkar
编码人快速骨骼肌球蛋白调节轻链 (HSRLC) 的 cDNA 克隆已从胎儿肌肉 cDNA 文库中分离出来并进行表征。该 cDNA 包含 170 个氨基酸 (aa) 的编码序列，5' 和 3' 非翻译区 (UTR) 分别包含 58 和 91 个核苷酸。 HSRLC 由人类基因组中的单拷贝基因编码，并在骨骼肌中显示出组织特异性的表达模式。 HSRLC 的衍生氨基酸序列与数据库序列的比较揭示了推定的钙结合区域中高度保守的 12 个氨基酸残基。 HSRLC 在所有 RLC 序列中是独一无二的，因为它具有三个连续的潜在可磷酸化丝氨酸残基。 HSRLC 的 Cys-129 对应于扇贝 RLC 的关键 Gly-117，这对其调节功能至关重要。疏水性残基簇被认为可以稳定 RLC 的 NH2 末端与肌球蛋白重链的结合，在 RLC 中表现出高度的序列保守性。除了通过诱变确定 HSRLC 功能研究的特定靶点外，这些结果还支持 RLC 基因进化而来的祖先基因的概念。
A cDNA clone encoding human fast skeletal myosin regulatory light chain (HSRLC) has been isolated and characterized from a fetal muscle cDNA library. The cDNA contains the coding sequence of 170 amino acids (aa) and 58 and 91 nucleotides in the 5′ and 3′ untranslated regions (UTRs), respectively. HSRLC is encoded by a single copy gene in the human genome and shows a tissue-specific pattern of expression in skeletal muscle. Comparison of derived amino acid sequence of HSRLC with database sequences reveals highly conserved 12 amino acid residues in a putative calcium-binding region. HSRLC is unique among all RLC sequences in having three consecutive potential phosphorylatable serine residues. The Cys-129 of HSRLC corresponds to the critical Gly-117 of scallop RLC that is essential for its regulatory function. The clusters of hydrophobic residues that are believed to stabilize the binding of NH2-terminal of RLC with myosin heavy chain show high sequence conservation in RLCs. Besides identifying specific targets for functional studies of HSRLC by mutagenesis, the results support the concept of an ancestral gene from which the RLC genes have evolved.