Follistatin specifically inhibits pituitary follicle stimulating hormone release in vitro.
Follistatin specifically inhibits pituitary follicle stimulating hormone release in vitro.
复制标题
卵泡抑素在体外特异性抑制垂体卵泡刺激激素的释放。
DOI：
10.1016/0006-291x(87)91614-7
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Ying,SY;Becker,A;Swanson,G;Tan,P;Ling,N;Esch,F;Ueno,N;Shimasaki,S;Guillemin,R
纯化的卵泡抑素的两种形式，一种分子量为35，000（35 Kd）的单链多肽蛋白，和一种分子量为32，000（32 Kd）的相关分子，其在糖基化或羧基末端截短方面不同于35 Kd形式，特异性地抑制大鼠垂体细胞原代培养物释放免疫反应性FSH。两种形式的卵泡抑素和卵泡抑素-A给出了相似的剂量-反应曲线，具有相同的斜率和最大效应，表明它们可能都通过相同的机制作用于垂体细胞。每种卵泡抑素的中位有效剂量（艾德50）为6.2-7.3 ng/ml（1.8× 10− 10 M），相当于卵泡抑素效价的1/3。35 Kd或32 Kd卵泡抑素的作用对抑制免疫反应性FSH的释放具有高度特异性，因为对其他垂体激素的分泌没有明显的伴随作用。卵泡抑素的作用，如卵泡抑素，是不同的下丘脑促垂体因子，需要在垂体单层培养系统中孵育18小时，以证明。卵泡抑素和卵泡抑素的共孵育显示了FSH释放抑制的累加效应。暴露于卵泡抑素的卵巢癌细胞的细胞内FSH消耗（0.01）显著低于用卵泡抑素处理的卵巢癌细胞，表明卵泡抑素可能主要作用于FSH释放的抑制，而不是像卵泡抑素那样同时作用于FSH的释放和合成。
Two forms of purified follistatin, a single-chain polypeptide of mol wt 35,000 (35 Kd) protein, and a related molecule of mol wt 32,000 (32 Kd), which differs from the 35 Kd form in glycosylation or carboxyl terminal truncation, specifically inhibit the release of immunoreactive FSH by primary cultures of rat pituitary cells. Both forms of follistatin and inhibin-A give similar dose-response curves, with identical slopes and maximal effects, suggesting that they may all act through the same mechanism on the pituitary cells. The median effective dose (ED 50) of each of the follistatins is 6.2–7.3 ng/ml (1.8× 10− 10 M), which corresponds to∼ 1 3 of the potency of inhibin. The effect of 35 Kd or 32 Kd follistatin is highly specific for suppressing the release of immunoreactive FSH since there is no demonstrable concomitant effect on the secretion of other pituitary hormones. The effect of follistatins, like that of inhibins, is different from that of the hypothalamic hypophysiotropic factors, requiring≧ 18 h of incubation in a pituitary monolayer culture system to demonstrate. Coincubation of inhibin and follistatin shows an additive effect in the suppression of FSH release. Pituitary cells exposed to follistatin have significantly less depletion of intracellular FSH (0.01) than those treated with inhibin, indicating that follistatin may act primarily on the suppression of FSH release rather than on both release and synthesis of FSH, as is the case with inhibin.