DNA based characterization of Ceratocystis fimbriata isolates associated with mango decline in Oman

DNA based characterization of Ceratocystis fimbriata isolates associated with mango decline in Oman
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基于 DNA 的与阿曼芒果衰退相关的 Ceratocystis fimbriata 分离株的表征

DOI:
10.1071/ap05080
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发表时间:
2005
影响因子:
1.4
通讯作者:
M. Wingfield
M. Wingfield
中科院分区:
农林科学4区
文献类型:
--
作者:
M. Wyk;A. Al;B. Wingfield;A. Al;M. Deadman;M. Wingfield

文献摘要

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摘要:阿曼苏丹国最近报道了一种严重的芒果衰退病。根据形态特征,病原菌先前被鉴定为Ceratocystis fimbriata。C.菌毛菌是巴西芒果最重要的病原体之一,它在阿曼的出现引起了人们的高度关注。最近的系统发育研究表明,C。fimbriata最有可能代表一个物种复合体。本研究的目的是根据DNA序列确认阿曼芒果病原体的身份。获得了内转录间隔区1和2区域以及5.8S rRNA基因区域的序列数据,并与C. fimbriata从几个主机和地理区域。来自阿曼的分离株被证明代表C. fimbriata sensu lato,并且也与来自巴西芒果的分离物最密切相关。这提供了一些证据,表明阿曼的芒果病原体可能起源于巴西。
Abstract.A serious mango decline disease has recently been reported from the Sultanate of Oman. Based on morphological characteristics, the pathogen responsible for the disease was previously identified as Ceratocystis fimbriata. C. fimbriata is one of the most important pathogens of mango in Brazil and its appearance in Oman is of great concern. Recent phylogenetic studies have shown that C. fimbriata most likely represents a species complex. The aim of this study was to confirm the identity of the mango pathogen in Oman based on DNA sequences. Sequence data were obtained for the internal transcribed spacer 1 and 2 regions and the 5.8S rRNA gene regions and these were compared with sequence data of C. fimbriata from several hosts and geographic areas. The isolates from Oman where shown to represent C. fimbriata sensu lato and also to be most closely related to an isolate from mango in Brazil. This provides some evidence that the mango pathogen in Oman might have originated in Brazil.