Multiple single unit recording in the cortex of monkeys using independently moveable microelectrodes
Multiple single unit recording in the cortex of monkeys using independently moveable microelectrodes
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DOI：
10.1016/s0165-0270(99)00121-1
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发表时间：
1999-12-15
影响因子：
3
通讯作者：
Lemon, RN
中科院分区：
文献类型：
--
作者：
Baker, SN;Philbin, N;Lemon, RN
从多个单神经元同时记录存在许多技术困难。然而，获得这样的数据具有许多优点，这使得克服技术问题非常值得。本报告描述了我们已经开发出的方法，允许清醒的行为猴子使用'埃克霍恩' 16电极微驱动器的记录。收集结构磁共振图像以指导电极放置。头部固定是通过使用一个专门设计的头盔，修改为多电极的方法，并通过一个新的记录室提供进入皮质。使用抗有丝分裂化合物减少暴露的硬脑膜上疤痕组织的生长。微驱动器的控制是通过一个计算机化的系统来实现的，该系统允许几个实验者同时移动不同的电极，大大减少了单个操作员的负担。神经元被识别为锥体束神经元通过逆行刺激通过长期植入电极;刺激控制集成到计算机化系统。最后，分析多个单个单元记录需要精确的方法来校正单元发射中的非平稳性。一种新的技术，这样的校正进行了讨论。(C)1999 Elsevier Science B. V.保留所有权利。
Simultaneous recording from multiple single neurones presents many technical difficulties. However, obtaining such data has many advantages, which make it highly worthwhile to overcome the technical problems. This report describes methods which we have developed to permit recordings in awake behaving monkeys using the 'Eckhorn' 16 electrode microdrive. Structural magnetic resonance images are collected to guide electrode placement. Head fixation is achieved using a specially designed headpiece, modified for the multiple electrode approach, and access to the cortex is provided via a novel recording chamber. Growth of scar tissue over the exposed dura mater is reduced using an anti-mitotic compound. Control of the microdrive is achieved by a computerised system which permits several experimenters to move different electrodes simultaneously, considerably reducing the load on an individual operator. Neurones are identified as pyramidal tract neurones by antidromic stimulation through chronically implanted electrodes; stimulus control is integrated into the computerised system. Finally, analysis of multiple single unit recordings requires accurate methods to correct for non-stationarity in unit firing. A novel technique for such correction is discussed. (C) 1999 Elsevier Science B.V. All rights reserved.