Response to darkness of late-responsive dark-inducible genes is positively regulated by leaf age and negatively regulated by calmodulin-antagonist-sensitive signalling in Arabidopsis thaliana
Response to darkness of late-responsive dark-inducible genes is positively regulated by leaf age and negatively regulated by calmodulin-antagonist-sensitive signalling in Arabidopsis thaliana
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DOI：
10.1093/pcp/pci174
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发表时间：
2005-10-01
影响因子：
4.9
通讯作者：
Nishida, I
中科院分区：
文献类型：
--
作者：
Fujiki, Y;Nakagawa, Y;Nishida, I
长时间黑暗后的诱导将拟南芥中的迟反应基因din 2和din 9与早反应基因din 3区分开来。在不同叶龄的莲座叶和早衰突变体中，前两个基因与衰老标记基因YLS 4同时被诱导。钙调素拮抗剂W-7，三氟拉嗪，氟奋乃静加速前基因在黑暗中的表达，但在光下没有，对后者的基因几乎没有影响。我们的研究结果表明，Ca 2 +/钙调素信号传递一个负信号，抑制延迟响应din基因的反应，延长黑暗。结果进行了讨论有关自然衰老。
Induction after prolonged darkness distinguishes the late-responsive genes din2 and din9 from the early-responsive gene din3 in Arabidopsis. The former genes were coincidently induced with the senescence marker gene YLS4 in rosette leaves of different ages and in the early-senescence mutant hys1. The calmodulin antagonists W-7, trifluoperazine, and fluphenazine accelerated the expression of the former genes in darkness but not in light, and had little effect on the latter gene. Our results suggest that Ca2+/ calmodulin signalling conveys a negative signal that suppresses the responses of late-responsive din genes to prolonged darkness. The results are discussed in relation to natural senescence.