Small cantilevers for force spectroscopy of single molecules
Small cantilevers for force spectroscopy of single molecules
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DOI：
10.1063/1.371039
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发表时间：
1999-08-15
影响因子：
3.2
通讯作者：
Hansma, PK
中科院分区：
文献类型：
--
作者：
Viani, MB;Schäffer, TE;Hansma, PK
我们已经用一个简单的工艺从氮化硅中制造出了小的矩形悬臂梁。它们的长度为9-50微米，宽度为3-5微米，厚度为86和102纳米。我们在一些悬臂端添加了金属反射板，以最大限度地提高反射率，同时将对温度变化的敏感度降至最低。我们通过小悬臂梁的热谱对其进行了表征，结果表明，在相同的弹性常数下，小悬臂梁比大悬臂梁能够测量更小的力，因为它们具有更低的粘性阻尼系数。最后，我们展示了小悬臂可以用于需要大测量带宽的实验，并且已经使用它们以比以前报道的传统悬臂快一个数量级的速度展开单个TiTiN分子。(C)1999年美国物理研究所。[S0021-8979(99)04916-6]。
We have used a simple process to fabricate small rectangular cantilevers out of silicon nitride. They have lengths of 9-50 mu m, widths of 3-5 mu m, and thicknesses of 86 and 102 nm. We have added metallic reflector pads to some of the cantilever ends to maximize reflectivity while minimizing sensitivity to temperature changes. We have characterized small cantilevers through their thermal spectra and show that they can measure smaller forces than larger cantilevers with the same spring constant because they have lower coefficients of viscous damping. Finally, we show that small cantilevers can be used for experiments requiring large measurement bandwidths, and have used them to unfold single titin molecules over an order of magnitude faster than previously reported with conventional cantilevers. (C) 1999 American Institute of Physics. [S0021-8979(99)04916-6].