Human apolipoprotein A-II: nucleotide sequence of a cloned cDNA, and localization of its structural gene on human chromosome 1.
Human apolipoprotein A-II: nucleotide sequence of a cloned cDNA, and localization of its structural gene on human chromosome 1.
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人载脂蛋白 A-II：克隆 cDNA 的核苷酸序列及其结构基因在人 1 号染色体上的定位。
DOI：
10.1016/0006-291x(84)90371-1
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发表时间：
1984
影响因子：
3.1
通讯作者：
Chan,L
中科院分区：
文献类型：
--
作者：
Moore,MN;Kao,FT;Tsao,YK;Chan,L
利用寡核苷酸杂交技术，分离出2个人载脂蛋白A-II的dscDNA克隆。其中一个克隆（pAII-1）已完成测序。它有433个核苷酸，其中包括一个由10个腺苷酸残基组成的聚a尾部，mRNA的所有编码区和3 ' -非翻译区以及部分5 ' -非翻译区。从cDNA克隆中得到的氨基酸序列包括100个氨基酸（包括23个氨基酸的前肽），这与Brewer等人报道的序列非常相似。国家的。学会科学。美国法典69,1304-1308)。[32 P]标记的pai -1作为染色体定位研究的探针，检测人-中国仓鼠杂交细胞中人类apoAII结构基因序列。对10个杂交种的Southern blot分析将该基因定位在人类1号染色体上。
Using the technique of oligonucleotide hybridization, we have isolated two dscDNA clones to human apolipoprotein A-II. One of the clones (pAII-1) has been completely sequenced. It has 433 nucleotides which includes a poly A tail of 10 adenylic acid residues, all the coding and 3′-non-translated regions of the mRNA and part of the 5′-non-translated region. The amino acid sequence derived from the cDNA clone includes 100 amino acids (including the 23 amino acid prepropeptide) which is very similar to the sequence reported by Brewer et al.(Proc. Natl. Acad. Sci. USA 69, 1304–1308).[32 P]-labeled pAII-1 was used as a probe in chromosome mapping studies to detect the human apoAII structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 10 hybrids localized the gene to human chromosome 1.