A gene-driven approach to the identification of ENU mutants in the mouse
A gene-driven approach to the identification of ENU mutants in the mouse
复制标题
DOI：
10.1038/ng847
复制
发表时间：
2002-03-01
期刊：
影响因子：
30.8
通讯作者：
Brown, SDM
中科院分区：
文献类型：
--
作者：
Coghill, EL;Hugill, A;Brown, SDM
构建来自用乙基亚硝基脲（ENU）诱变的小鼠的DNA和精子的平行档案代表了用于鉴定小鼠基因组中任何基因中的点突变的潜在强大且快速的方法。我们提供这种方法的支持，并报告识别的基因（Gjb2）编码连接蛋白26的突变，使用档案建立从英国ENU诱变程序。
The construction of parallel archives of DNA and sperm from mice mutagenized with ethylnitrosurea (ENU) represents a potentially powerful and rapid approach for identifying point mutations in any gene in the mouse genome. We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program.