The Drosophila Transcription Factor Ultrabithorax Self-Assembles into Protein-Based Biomaterials with Multiple Morphologies
The Drosophila Transcription Factor Ultrabithorax Self-Assembles into Protein-Based Biomaterials with Multiple Morphologies
复制标题
DOI：
10.1021/bm801315v
复制
发表时间：
2009-04-01
影响因子：
6.2
通讯作者：
Bondos, Sarah E.
中科院分区：
文献类型：
--
作者：
Greer, Alexandra M.;Huang, Zhao;Bondos, Sarah E.
使用蛋白质作为材料组装的单体可以实现化学、物理和功能特性的定制。然而，天然材料形成蛋白很难以重组蛋白单体的形式生产，并且需要苛刻的条件来启动组装。我们使用重组转录因子 Ultrabithorax 生成了材料，Ultrabithorax 是一种果蝇蛋白，未知或预计不会在体内形成延伸的寡聚体。 Ultrabithorax 在空气-水界面处自组装成纳米级纤维，进一步结合形成宏观薄膜、片材、绳索和束缚胶囊。这些材料具有自粘性，可以建造更复杂的建筑。 Ultrabithorax 序列包含两个能够生成材料的区域，其中只有一个包含弹性蛋白中发现的基序。然而，必须包括两个最小区域才能生产坚固的材料。与其他基于蛋白质的材料相比，Ultrabithorax 的组装浓度显着降低，时间尺度更快，条件更温和，其特性有利于未来的材料工程和功能化。
The use of proteins as monomers for materials assembly enables customization of chemical, physical, and functional properties. However, natural materials-forming proteins are difficult to produce as recombinant protein monomers and require harsh conditions to initiate assembly. We have generated materials using the recombinant transcription factor Ultrabithorax, a Drosophila melanogaster protein not known or anticipated to form extended oligomers in vivo. Ultrabithorax self-assembles at the air-water interface into nanoscale fibers, which further associate to form macroscale films, sheets, ropes, and tethered encapsulates. These materials self-adhere, allowing construction of more complex architectures. The Ultrabithorax sequence contains two regions capable of generating materials, only one of which contains motifs found in elastomeric proteins. However, both minimal regions must be included to produce robust materials. Relative to other protein-based materials, Ultrabithorax assembles at significantly reduced concentrations, on faster timescales, and under gentler conditions, properties that facilitate future materials engineering and functionalization.