Low levels of apoptosis and high FLIP expression in early rheumatoid arthritis synovium
Low levels of apoptosis and high FLIP expression in early rheumatoid arthritis synovium
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DOI：
10.1136/ard.61.10.934
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发表时间：
2002-10-01
影响因子：
27.4
通讯作者：
Klareskog, L
中科院分区：
文献类型：
--
作者：
Catrina, AI;Ulfgren, AK;Klareskog, L
目的：定义滑膜细胞凋亡的病程，炎症细胞类型，FLIP（FLICE样抑制蛋白），细胞因子的表达在患者中，类风湿关节炎（RA）hemodynamics：滑膜活检标本11例长期RA（中位病程21年）和8例早期RA（中位病程5个月）进行了研究。评价细胞凋亡（TUNEL法结合形态学分析）、细胞表面标志物（CD 3、CD 68）、细胞因子（白细胞介素（IL）1 α、IL 1 β、肿瘤坏死因子α和IL 6）和FLIP表达。计算机辅助图像分析用于定量。长期RA组滑膜细胞凋亡水平明显高于早期RA组（8.8%v0.6%，p=0.001），而巨噬细胞数量和FLIP表达在早期疾病组高于长期RA组（分别为16.2%对8.3%，p=0.02和31.1%对0.2%，p=0.001）。所有三种标记物与疾病持续时间显著相关（R=-0.7，p
Objectives: To define synovial apoptosis with respect to disease duration, inflammatory cell type, FLIP (FLICE-like inhibitory protein), and cytokines expression in patients,with rheumatoid arthritis (RA).Methods: Synovial biopsy specimens from 11 patients with longstanding RA (median disease duration 21 years) and eight with early RA (median disease duration five months) were investigated. Apoptosis (TUNEL method combined with morphological analysis), cell surface markers (CD3, CD68), cytokines (interleukin (IL) 1alpha, IL1beta, tumour necrosis factor alpha, and IL6), and FLIP expression were evaluated. Computer assisted image analysis was used for quantification.Results: The apoptosis level in RA synovium was significantly higher in the group of patients with longstanding RA than in the patients with early RA (8.8% v 0.6%, p=0.001), while the number of macrophages and FLIP expression were higher in the group with early disease than in the group with longstanding RA (16.2% v 8.3%, p=0.02 and 31.1% v 0.2%, p=0.001 respectively). All three markers correlated significantly with disease duration (R=-0.7, p