GENOTYPE AT THE P450SCC LOCUS DETERMINES DIFFERENCES IN THE AMOUNT OF P450SCC PROTEIN AND MAXIMAL TESTOSTERONE PRODUCTION IN MOUSE LEYDIG-CELLS
GENOTYPE AT THE P450SCC LOCUS DETERMINES DIFFERENCES IN THE AMOUNT OF P450SCC PROTEIN AND MAXIMAL TESTOSTERONE PRODUCTION IN MOUSE LEYDIG-CELLS
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DOI：
10.1210/mend-4-10-1459
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发表时间：
1990-10-01
影响因子：
--
通讯作者：
PAYNE, AH
中科院分区：
文献类型：
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作者：
NOLAN, CJ;PAYNE, AH
Leydig细胞中最大睾酮产生的遗传差异与P450 SCC基因座的基因型差异有关。P450 SCC基因之间的遗传关系，Leydig细胞P450 SCC蛋白的量，和最大的睾酮产生的小鼠中确定的F2代来自SWR/J小鼠（SWR），一个高Leydig细胞睾酮产生的菌株，和C3 H/HeJ（C3 H），一个低Leydig细胞睾酮产生的菌株。结果表明，在SHR和C3 H小鼠间P450 SCC基因存在限制性片段长度多态性。该限制性片段长度多态性用于鉴定P450 SCC基因的SWR或C3 H等位基因纯合的F2小鼠。比较两种类型的纯合小鼠的最大睾酮产生和P450 SCC，P45017 α，3. beta.-羟基类固醇脱氢酶异构酶（3 β HSD）蛋白。SWR中的最大睾酮产生、P450 SCC和3 β HSD的量显著大于33 H祖小鼠。在F2小鼠中，P450 SCC的SWR或C3 H等位基因的纯合子，最大睾酮产生和P450 SCC蛋白量的差异与两个祖先品系的差异相当。P450 SCC蛋白表达量与最大睾酮分泌量呈显著正相关（r = 0.75; P < 0.01）。P45017 α的量无差异。或3 β HSD。这些结果表明，基因型在P450 SCC位点，这是以前映射到小鼠9号染色体，确定了间质细胞P450 SCC蛋白的量，和P450 SCC蛋白的量是一个重要的决定因素间质细胞的能力，在这些菌株中的睾酮生产。
A genetic difference in maximal testosterone production in Leydig cells relates to differences in the genotype at the P450scc locus. The genetic relationship between the P450scc gene, the amount of Leydig cell P450scc protein, and maximal testosterone production was determined in the F2 generation of mice derived from SWR/J mice (SWR), a high Leydig cell testosterone-producing strain, and from C3H/HeJ (C3H), a low Leydig cell testosterone-producing strain. A restriction fragment length polymorphism was identified in the P450scc gene between SWR and C3H mice. This restriction fragment length polymorphism was used to identify F2 mice homozygous for the SWR or the C3H alleles of the P450scc gene. The two types of homozygous mice were compared with regard to maximal testosterone production and the amounts of P450scc, P45017.alpha., and 3.beta.-hydroxysteroid dehydrogenase isomerase (3.beta.HSD) proteins. Maximal testosterone production, amounts of P450scc and 3.beta.HSD were significantly greater in the SWR than in the 33H progenitor mice. In the F2 mice, homozygous for either the SWR or the C3H allele of P450scc, the differences in maximal testosterone production and the amount of P450scc protein were comparable to the differences in the two progenitor strains. A significant correlation (r = 0.75; P < 0.01) was found between the amount of P450scc protein and maximal testosterone production. No differences in the amounts of P45017.alpha. or 3.beta.HSD were observed in the F2 males. These results demonstrate that the genotype at the P450scc locus, which were previously mapped to mouse chromosome 9, determined the amount of Leydig cell P450scc protein and that the amount of p450scc protein is an important determinant of Leydig cell capacity for testosterone production in these strains.