Phospholipids in lipoproteins: compositional differences across VLDL, LDL, and HDL in pregnant women
Phospholipids in lipoproteins: compositional differences across VLDL, LDL, and HDL in pregnant women
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DOI：
10.1186/s12944-019-0957-z
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发表时间：
2019-01-22
影响因子：
4.5
通讯作者：
Koletzko, Berthold
中科院分区：
文献类型：
--
作者：
Rauschert, Sebastian;Gazquez, Antonio;Koletzko, Berthold
目的分析瘦孕妇和肥胖孕妇极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）单层磷脂组成的差异。方法从10例瘦肉孕妇和10例肥胖孕妇血浆中分离sldl、HDL和VLDL，采用液相色谱-串联质谱法分析其磷脂酰胆碱（PC）和鞘磷脂（SM）的种类组成。使用Wilcoxon-Mann-Whitney U检验和主成分分析（PCA）来研究瘦/肥胖组和脂蛋白种类之间的代谢物谱是否存在差异。结果肥胖孕妇与非肥胖孕妇的代谢物水平无显著差异。PCA成分1和2在低密度脂蛋白、高密度脂蛋白和VLDL之间分离，但在正常体重和肥胖女性之间不分离。12个SM和1个PCae在LDL中比在VLDL中更丰富。相比之下，4个酰基-烷基- pc和2个二酰基- pc在HDL中的含量明显高于LDL。3个SM、7个酰基-烷基- pc和1个二酰基- pc （HDL较高）和13个SM （VLDL较高）的VLDL和HDL存在差异。我们还发现一些磷脂种类与高密度脂蛋白和低密度脂蛋白胆固醇有关。结论孕妇的HDL、LDL和VLDL的磷脂组成差异显著，与先前在男性和非孕妇中的发现相似。肥胖和瘦弱孕妇的脂蛋白相关代谢物谱没有显著差异。
ObjectiveThe aim of this study was to analyse the differences in the phospholipid composition of very low density (VLDL), low density (LDL) and high density lipoprotein (HDL) monolayers in pregnant lean and obese women.MethodsLDL, HDL, and VLDL were isolated from plasma samples of 10 lean and 10 obese pregnant women, and their species composition of phosphatidylcholines (PC) and sphingomyelins (SM) was analysed by liquid-chromatography tandem mass-spectrometry. Wilcoxon-Mann-Whitney U test and principal component analysis (PCA) were used to investigate if metabolite profiles differed between the lean/obese group and between lipoprotein species.ResultsNo significant differences have been found in the metabolite levels between obese and non-obese pregnant women. The PCA components 1 and 2 separated between LDL, HDL, and VLDL but not between normal weight and obese women. Twelve SM and one PCae were more abundant in LDL than in VLDL. In contrast, four acyl-alkyl-PC and two diacyl-PC were significantly higher in HDL compared to LDL. VLDL and HDL differed in three SM, seven acyl-alkyl-PC and one diacyl-PC (higher values in HDL) and 13 SM (higher in VLDL). We also found associations of some phospholipid species with HDL and LDL cholesterol.ConclusionIn pregnant women phospholipid composition differs significantly in HDL, LDL and VLDL, similar to previous findings in men and non-pregnant women. Obese and lean pregnant women showed no significant differences in their lipoprotein associated metabolite profile.