EFFECTS OF BRAIN-DERIVED NEUROTROPHIC FACTOR ON OPTIC AXON BRANCHING AND REMODELING IN-VIVO
EFFECTS OF BRAIN-DERIVED NEUROTROPHIC FACTOR ON OPTIC AXON BRANCHING AND REMODELING IN-VIVO
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DOI：
10.1038/378192a0
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发表时间：
1995-11-09
期刊：
影响因子：
64.8
通讯作者：
FRASER, SE
中科院分区：
文献类型：
--
作者：
COHENCORY, S;FRASER, SE
神经营养素类被认为对脊椎动物神经元的存活和分化很重要（1）。基于它们在神经元神经支配时在靶组织中的表达（2，3）以及它们对轴突发芽的影响（4-6），已经提出了靶源性神经营养因子的作用。然而，直接在体内证据，他们参与轴突树枝化仍然难以捉摸。我们已经在体内显微镜下，随着时间的推移，个别视神经轴突，并已检查了神经营养的脑源性神经营养因子（BDNF）在其发展中的作用。在这里，我们表明，注射BDNF到视顶盖的活爪蟾蝌蚪增加了分支和复杂性的视神经轴突终端乔木。相反，注射BDNF特异性中和抗体减少了轴突分支和复杂性。这些效应的发生迅速（2小时内），并持续整个24小时观察期。其他神经营养素几乎没有或没有显着的影响。这些结果表明，参与轴突终端的动态制作的神经营养因子，并建议在发育中的中枢神经系统的突触图案的直接作用，靶源性BDNF。
NEUROTROPHINS are thought to be important for the survival and differentiation of vertebrate neurons(1). Roles have been suggested for target-derived neurotrophins, based both on their expression in target tissues at the time of neuron innervation(2,3), and on their effects on axonal sprouting(4-6). However, direct in vivo evidence of their involvement in axon arborization has remained elusive. We have used in vivo microscopy to follow individual optic axons over time, and have examined the role of the neurotrophin brain-derived neurotrophic factor (BDNF) in their development. Here we show that injection of BDNF into the optic tectum of live Xenopus laevis tadpoles increased the branching and complexity of optic axon terminal arbors. In contrast, injection of specific neutralizing antibodies to BDNF reduced axon arborization and complexity. The onset of these effects was rapid (within 2 hours) and persisted throughout the 24-hour observation period. Other neurotrophins had little or no significant effects. These results demonstrate the involvement of neurotrophins in the dynamic elaboration of axon terminals, and suggest a direct role for target-derived BDNF during synaptic patterning in the developing central nervous system.