Splicing of pre-mRNA: mechanism, regulation and role in development.
Splicing of pre-mRNA: mechanism, regulation and role in development.
复制标题
DOI：
10.1016/0959-437x(93)90093-5
复制
发表时间：
1993
影响因子：
4
通讯作者：
D. Rio
中科院分区：
文献类型：
--
作者：
D. Rio
在过去的一年里，在理解RNA结合因子如何促进剪接位点选择和剪接体组装，并赋予前mRNA剪接反应的保真度方面取得了重大进展。此外，一些研究揭示了剪接体中RNA-RNA相互作用的复杂网络，加强了核前mRNA剪接与自剪接I组和II组内含子之间的结构和功能上的相似性。这些新数据进一步支持了前mRNA剪接是通过RNA介导的催化发生的这一观点，并相当戏剧性地说明了剪接体周期中构象变化的动态性质。关于组织特异性的Pre-mRNA剪接，一些研究已经开始阐明控制剪接位点选择的潜在机制，以及所谓的“一般”RNA结合蛋白，如异质性核糖核蛋白，如何参与决定不同的剪接模式。最后，一个涉及剪接在发育中的作用的新主题是，不同类型的细胞可以通过不同的剪接模式触发不同的转录程序，这些剪接模式产生具有不同活性或DNA结合特异性的转录因子亚型。
Over the past year, significant progress has been made in the understanding of how RNA-binding factors may facilitate splice-site selection and spliceosome assembly, and confer fidelity to the pre-mRNA splicing reaction. In addition, a number of studies have revealed a complex network of RNA-RNA interactions in the spliceosome, strengthening the structural and functional parallels between nuclear pre-mRNA splicing and the self-splicing group I and group II introns. These new data further support the idea that pre-mRNA splicing occurs by RNA-mediated catalysis and illustrate quite dramatically the dynamic nature of conformational changes in the spliceosome cycle. With respect to tissue-specific pre-mRNA splicing, a number of studies have begun to illuminate mechanisms underlying control of splice-site selection and how so-called ‘general’ RNA-binding proteins, such as heterogeneous nuclear ribonucleoproteins, may be involved in determining different splicing patterns. Finally, an emerging theme involving the role of splicing in development is that differential transcriptional programs can be triggered in different cell types by alternative splicing patterns that generate transcription factor isoforms with different activities or DNA-binding specificities.