CD8+γδ T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response
CD8+γδ T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response
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DOI：
10.1155/2019/6348060
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发表时间：
2019-12-06
影响因子：
4.3
通讯作者：
Uhlin, Michael
中科院分区：
文献类型：
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作者：
Gaballa, Ahmed;Arruda, Lucas C. M.;Uhlin, Michael
γ δ（gamma delta）T细胞在人巨细胞病毒（HCMV）免疫监视中的作用多年来一直是研究兴趣的焦点。最近的报道已经显示γ δ T细胞响应于HCMV的大量克隆增殖，揭示了γ δ T细胞的适应性免疫应答。然而，大多数的努力都集中在V δ 2（阴性）γ δ T细胞亚群，而较少注意到研究其他不太常见的γ δ T细胞亚群。在这方面，一个独特的亚群的γ δ T细胞，表达的CD 8辅助受体（CD 8（+）γ δ T细胞）还没有得到彻底的探讨。它是否涉及HCMV应答及其产生适应性应答的能力尚未得到彻底研究。在这项研究中，我们结合流式细胞术和免疫测序的TCR γ链（TRG），深入分析骨髓（BM）移植γ δ T细胞的CMV血清阳性（CMV+）和CMV血清阴性（CMV-）的捐助者。我们发现，与CMV-移植物相比，CMV+移植物中CD 8（+）γ δ T细胞的频率显著更高（P
The role of gamma delta (gamma delta) T cells in human cytomegalovirus (HCMV) immune surveillance has been the focus of research interest for years. Recent reports have shown a substantial clonal proliferation of gamma delta T cells in response to HCMV, shedding light on the adaptive immune response of gamma delta T cells. Nevertheless, most efforts have focused on V delta 2(neg)gamma delta T cell subset while less attention has been given to investigate other less common gamma delta T cell subsets. In this regard, a distinct subpopulation of gamma delta T cells that expresses the CD8 coreceptor (CD8(+)gamma delta T cells) has not been thoroughly explored. Whether it is implicated in HCMV response and its ability to generate adaptive response has not been thoroughly investigated. In this study, we combined flow cytometry and immune sequencing of the TCR gamma-chain (TRG) to analyze in-depth bone marrow (BM) graft gamma delta T cells from CMV seropositive (CMV+) and CMV seronegative (CMV-) donors. We showed that the frequency of CD8(+)gamma delta T cells was significantly higher in CMV+ grafts compared to CMV- grafts (P