CYCLIN-G IS A TRANSCRIPTIONAL TARGET OF THE P53 TUMOR-SUPPRESSOR PROTEIN
CYCLIN-G IS A TRANSCRIPTIONAL TARGET OF THE P53 TUMOR-SUPPRESSOR PROTEIN
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DOI：
10.1002/j.1460-2075.1994.tb06807.x
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发表时间：
1994-10-17
期刊：
影响因子：
11.4
通讯作者：
BEACH, D
中科院分区：
文献类型：
--
作者：
OKAMOTO, K;BEACH, D
通过基于PCR的差异筛选方法，cyclin G被鉴定为p53抑癌基因产物的新转录靶点。在小鼠 p53 温度敏感白血病细胞系和小鼠胚胎成纤维细胞 (MEF) 中，γ 射线照射后，细胞周期蛋白 G mRNA 被迅速诱导。 p53 缺陷小鼠的 MEF 表达的细胞周期蛋白 G 水平比野生型小鼠低 10 倍以上。使用 DNA 结合测定，在细胞周期蛋白 G 基因上游鉴定出特定的 p53 结合位点，该位点在瞬时转染测定中充当 p53 依赖性顺式作用元件。这些结果表明，cyclin G 可能参与 p53 介导的途径来预防肿瘤发生。
Through a PCR-based differential screening method, cyclin G was identified as a novel transcriptional target of the p53 tumor suppressor gene product. In both a mouse p53 temperature-sensitive leukemic cell line and mouse embryonic fibroblasts (MEF) after gamma-irradiation, cyclin G mRNA was rapidly induced. MEF from a p53-deficient mouse expressed cyclin G at a level >10-fold lower than that from a wild-type mouse. Using a DNA binding assay, a specific p53 binding site was identified upstream from the cyclin G gene, which functioned as a p53-dependent cis-acting element in a transient transfection assay. These results suggest that cyclin G might participate in a p53-mediated pathway to prevent tumorigenesis.