Growth, Nitrogen Fixation, and Spectral Attenuation in Cultivated Trichodesmium Species
Growth, Nitrogen Fixation, and Spectral Attenuation in Cultivated Trichodesmium Species
复制标题
栽培毛藻物种的生长、固氮和光谱衰减
DOI：
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发表时间：
1993
影响因子：
4.4
通讯作者：
C. Lassen
中科院分区：
文献类型：
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作者：
L. Prufert;H. Paerl;C. Lassen
束毛藻属的生理研究一直受到维持活跃生长、固氮培养物的困难的阻碍。以前的种植成功没有被广泛复制。本文介绍了一种简单的改良海水培养基和处理技术，用于在实验室培养中维持Thiebautii束毛藻的活跃生长超过1年。培养的人口，从沿海大西洋沃茨分离，具有0.23分裂天-1的增长率，并在指数增长过程中表现出光依赖性固氮。在生长过程中存在各种形态，包括单生毛状体和聚集体（球形蓬松和纺锤状簇）。光谱和标量辐照度测量自然发生的（沿海大西洋）聚集与小（直径，50至70 μm）球形光纤传感器。与自然发生的泡芙，栽培束毛藻聚集体表现出符合低光适应的光谱特性。培养的膨化型聚集体也进行了检查，通过使用氧微电极。用于培养的简单培养基和方法应易于重现，并适于进一步的操作和修改，可用于束毛藻属的生理研究。在文化中。
Physiological studies of Trichodesmium species have been hindered by difficulties in maintaining actively growing, nitrogen-fixing cultures. Previous cultivation successes have not been widely duplicated. We present here a simple modified seawater medium and handling techniques which have been used to maintain actively growing Trichodesmium thiebautii in laboratory culture for over 1 year. The cultured population, isolated from coastal Atlantic waters, has a growth rate of 0.23 division day-1 and exhibits light-dependent nitrogen fixation during exponential growth. Various morphologies, including solitary trichomes, and aggregates (spherical puffs and fusiform tufts) are present during growth. Spectral and scalar irradiance were measured within naturally occurring (coastal Atlantic) aggregates with small (diameter, 50 to 70 μm) spherical fiber-optic sensors. In contrast to naturally occurring puffs, cultivated Trichodesmium aggregates exhibited spectral properties consistent with low-light adaptation. Cultivated puff-type aggregates were also examined by using oxygen microelectrodes. The simple medium and approach used for cultivation should be easily reproducible and amenable to further manipulations and modifications useful for physiological studies of Trichodesmium spp. in culture.