FgSKN7 and FgATF1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in Fusarium graminearum
FgSKN7 and FgATF1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in Fusarium graminearum
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FgSKN7 和 FgATF1 在禾谷镰刀菌子囊孢子发生、发病机制和应激反应中具有重叠功能
DOI：
10.1111/1462-2920.12561
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发表时间：
2015-04-01
影响因子：
5.1
通讯作者：
Xu, Jin-Rong
中科院分区：
文献类型：
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作者：
Jiang, Cong;Zhang, Shijie;Xu, Jin-Rong
由禾谷镰刀菌引起的赤霉病是小麦和大麦最具破坏性的病害之一。该病原菌产生的脱氧雪腐镰刀菌烯醇（DON）是一种重要的霉菌毒素和毒力因子。由于氧化爆发是一种常见的防御反应，并且活性氧 (ROS) 会诱导 DON 产生，因此在本研究中，我们表征了三个与应激相关的转录因子基因 FgAP1、FgATF1 和 FgSKN7 的功能关系。尽管它们都在氧化应激耐受中发挥作用，但 FgAP1 或 FgATF1 的缺失对 DON 的产生没有显着影响。相比之下，Fgskn7 突变体的 DON 产量减少，并且 H2O2 诱导的 TRI 基因表达存在缺陷。 Fgap1 突变体除了对 H2O2 的敏感性增加之外没有可检测的表型，并且 Fgap1Fgatf1 和 Fgap1Fgskn7 突变体比单个突变体缺乏额外或更严重的表型。 Fgatf1（而非 Fgskn7）突变体的毒力显着降低，子囊孢子释放延迟。 Fgskn7Fgatf1双突变体比Fgatf1或Fgskn7突变体在生长、分生孢子和毒力方面具有更严重的缺陷。它没有产生四细胞子囊孢子，而是在每个子囊中形成八个小的单细胞子囊孢子。因此，FgSKN7 和 FgATF1 在禾谷镰刀菌生长、发育和发病机制的细胞内 ROS 信号传导中必须具有重叠的功能。
Fusarium head blight caused by Fusarium graminearum is one of the most destructive diseases of wheat and barley. Deoxynivalenol (DON) produced by the pathogen is an important mycotoxins and virulence factor. Because oxidative burst is a common defense response and reactive oxygen species (ROS) induces DON production, in this study, we characterized functional relationships of three stress-related transcription factor genes FgAP1, FgATF1 and FgSKN7. Although all of them played a role in tolerance to oxidative stress, deletion of FgAP1 or FgATF1 had no significant effect on DON production. In contrast, Fgskn7 mutants were reduced in DON production and defective in H2O2-induced TRI gene expression. The Fgap1 mutant had no detectable phenotype other than increased sensitivity to H2O2 and Fgap1Fgatf1 and Fgap1Fgskn7 mutants lacked additional or more severe phenotypes than the single mutants. The Fgatf1, but not Fgskn7, mutant was significantly reduced in virulence and delayed in ascospore release. The Fgskn7Fgatf1 double mutant had more severe defects in growth, conidiation and virulence than the Fgatf1 or Fgskn7 mutant. Instead of producing four-celled ascospores, it formed eight small, single-celled ascospores in each ascus. Therefore, FgSKN7 and FgATF1 must have overlapping functions in intracellular ROS signalling for growth, development and pathogenesis in F.graminearum.