High-expression of ZBP-89 correlates with distal metastasis and poor prognosis of patients in clear cell renal cell carcinoma
High-expression of ZBP-89 correlates with distal metastasis and poor prognosis of patients in clear cell renal cell carcinoma
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ZBP-89的高表达与透明细胞肾细胞癌患者的远端转移和不良预后相关
DOI：
10.1016/j.bbrc.2012.08.146
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发表时间：
2012-10-05
影响因子：
3.1
通讯作者：
Cao, Yun
中科院分区：
文献类型：
--
作者：
Cai, Mu-Yan;Luo, Rong-Zhen;Cao, Yun
ZBP-89是一种Kruppe型锌指转录因子，参与肿瘤的发生、侵袭和转移。然而，ZBP-89在肾透明细胞癌(CcRCC)中的表达状况仍不清楚。应用实时定量聚合酶链式反应和Western Blot检测发现，在新鲜癌组织中，ZBP-89在mRNA和蛋白水平分别有79.2%(19/24)和83.3%(5/6)的ccRCC表达显著下调。免疫组织化学显示ZBP-89在肾细胞癌组织中的表达显著降低，在73.9%(105/142)的肿瘤组织中低表达，而在癌旁肾组织中的低表达为48.1%(52/108)。此外，ZBP-89的表达与肾细胞癌的临床病理特征有关，包括TNM分期(P=0.005)和远处转移(P=0.001)。进一步研究证实，转移性肾细胞癌组织中ZBP-的表达明显高于非转移性肾细胞癌组织(P=0.002)。此外，单因素分析显示，低表达ZBP-89的肾细胞癌患者的生存期比高表达的患者更长(P<；0.0001)。更重要的是，多因素分析显示ZBP-89是总生存期的独立预测因子(HR，2.871；95%CI，1.409-5.853；P=0.004)。总之，我们的研究提供了强有力的证据表明，ZBP-89在肾细胞癌中显著下调，有望成为预测肾细胞癌患者远处转移和预后的生物标志物。(C)2012 Elsevier Inc.保留所有权利。
ZBP-89, a Kruppel-type zinc-finger transcription factor, is found to participate in tumor development, invasion and metastasis. However, the expression status of ZBP-89 in clear cell renal cell carcinoma (CCRCC) remains elusive. Using quantitative real-time-PCR and Western Blot, we found that, in fresh cancer tissues, ZBP-89 was remarkably decreased in 79.2% (19/24) and 83.3% (5/6) of CCRCC at mRNA and protein level, respectively. Immunohistochemistry also revealed a significant decline of ZBP-89 expression in CCRCC, showing that low expression of ZBP-89 was present in 73.9% (105/142) of tumorous tissues but in 48.1% (52/108) of the corresponding adjacent kidney tissues. Furthermore, ZBP-89 expression in CCRCC was significantly correlated with several clinicopathological features, including TNM stage (P = 0.005) and distal metastasis (P = 0.001). Further study confirmed that ZBP-89 expression was markedly higher in metastatic CCRCC than that in non-metastatic tissue (P = 0.002). In addition, CCRCC patients with low ZBP-89 expression survived longer than those with high ZBP-89 expression, as indicated by the result of univariate analysis (P < 0.0001). More importantly, multivariate analysis revealed that ZBP-89 was an independent predictor of overall survival (HR, 2.871; 95% Cl, 1.409-5.853; P = 0.004). Collectively, our study provides vigorous evidence that ZBP-89 was significantly downregulated in CCRCC and could be served as a promising biomarker for prediction of distal metastasis and prognosis of patient with CCRCC. (C) 2012 Elsevier Inc. All rights reserved.