Inhibition of protein synthesis prevents cell death in sensory and parasympathetic neurons deprived of neurotrophic factor in vitro.
Inhibition of protein synthesis prevents cell death in sensory and parasympathetic neurons deprived of neurotrophic factor in vitro.
复制标题
抑制蛋白质合成可防止体外缺乏神经营养因子的感觉和副交感神经元的细胞死亡。
DOI：
10.1002/neu.480210410
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Davies,AM
中科院分区：
文献类型：
--
作者：
Scott,SA;Davies,AM
在发育中的脊椎动物神经系统中，神经元开始支配它们的靶标后不久，它们就变得依赖神经营养因子的供应来生存，例如神经生长因子(NGF)。最近，Martin等人(1988)表明，抑制蛋白质合成可以阻止NGF剥夺的交感神经元的死亡，这表明NGF通过抑制活跃的细胞死亡程序来促进神经元的存活。为了确定其他神经营养因子是否可以通过类似的机制调控神经元的存活，我们检测了其他需要不同神经营养因子的胚胎神经元群体中抑制蛋白质和RNA合成的效果，这些神经元是：(1)三叉神经中脑神经元，一组由脑源性神经营养因子支持的本体感受神经元；(2)三叉神经节背内侧神经元，一组由NGF支持的皮肤感觉神经元；(3)睫状节神经元，一组由睫状神经营养因子支持的副交感神经元。在体外，阻断蛋白质或RNA的合成都能将三种神经元从神经营养因子剥夺所致的细胞死亡中拯救出来。因此，至少有三种不同的神经营养因子似乎通过类似的机制促进存活，这可能涉及到抑制内源性细胞死亡程序。
Shortly after neurons begin to innervate their targets in the developing vertebrate nervous system they become dependent on the supply of a neurotrophic factor, such as nerve growth factor (NGF) for survival. Recently, Martin et al.(1988) have shown that inhibiting protein synthesis prevents the death of NGF-deprived sympathetic neurons, suggesting that NGF promotes neuronal survival by suppressing an active cell death program. To determine if other neurotrophic factors may regulate neuronal survival by a similar mechanism we examined the effects of inhibiting protein and RNA synthesis in other populations of embryonic neurons that require different neurotrophic factors, namely:(1) trigeminal mesencephalic neurons, a population of proprioceptive neurons that are supported by brain-derived neurotrophic factor;(2) dorsomedial trigeminal ganglion neurons, a population of cutaneous sensory neurons that are supported by NGF;(3) and ciliary ganglion neurons, a population of parasympathetic neurons that are supported by ciliary neuronotrophic factor. Blocking either protein or RNA synthesis rescued all three populations of neurons from cell death induced by neurotrophic factor deprivation in vitro. Thus, at least three different neurotrophic factors appear to promote survival by a similar mechanism that may involve the suppression of an endogenous cell death program.