Expression profiling of mammalian male meiosis and gametogenesis identifies novel candidate genes for roles in the regulation of fertility
Expression profiling of mammalian male meiosis and gametogenesis identifies novel candidate genes for roles in the regulation of fertility
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DOI：
10.1091/mbc.e03-10-0762
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发表时间：
2004-03-01
影响因子：
3.3
通讯作者：
Primig, M
中科院分区：
文献类型：
--
作者：
Schlecht, U;Demougin, P;Primig, M
我们报告了通过使用高密度寡核苷酸微阵列和高度富集的细胞群对经历有丝分裂生长、减数分裂和配子发生的哺乳动物雄性生殖细胞进行全面的大规模表达谱分析。在研究的 11,955 个大鼠基因座中，与总睾丸、体细胞支持细胞以及大脑和骨骼肌对照相比，有 1268 个基因座被鉴定为在随后发育阶段的生殖细胞中存在差异转录。这些基因座被组织成四个表达簇，分别对应于体细胞、有丝分裂、减数分裂和减数分裂后细胞类型。这项工作提供了有关已知在雄性生殖细胞发育过程中重要的 200 种类似基因的表达模式的信息。其中大约 40 个包含在一组 121 个转录本中，我们报告了三种体细胞控制细胞类型中生殖细胞的表达和转录的缺乏。此外，我们还证明了 293 个尚未表征的转录物在有丝分裂、减数分裂和/或减数分裂后生殖细胞中的睾丸表达和转录诱导，其中一些可能编码参与精子发生和生育力的因子。该组还包含创新避孕药的潜在生殖细胞特异性靶标。可通过 http://www.germonline.org 上的 GermOnline 数据库方便地访问数据的图形显示。
We report a comprehensive large-scale expression profiling analysis of mammalian male germ cells undergoing mitotic growth, meiosis, and gametogenesis by using high-density oligonucleotide microarrays and highly enriched cell populations. Among 11,955 rat loci investigated, 1268 were identified as differentially transcribed in germ cells at subsequent developmental stages compared with total testis, somatic Sertoli cells as well as brain and skeletal muscle controls. The loci were organized into four expression clusters that correspond to somatic, mitotic, meiotic, and postmeiotic cell types. This work provides information about expression patterns of similar to200 genes known to be important during male germ cell development. Approximately 40 of those are included in a group of 121 transcripts for which we report germ cell expression and lack of transcription in three somatic control cell types. Moreover, we demonstrate the testicular expression and transcriptional induction in mitotic, meiotic, and/or postmeiotic germ cells of 293 as yet uncharacterized transcripts, some of which are likely to encode factors involved in spermatogenesis and fertility. This group also contains potential germ cell-specific targets for innovative contraceptives. A graphical display of the data is conveniently accessible through the GermOnline database at http://www.germonline.org.