Carbohydrate-active enzymes involved in the secondary cell wall biogenesis in hybrid aspen
Carbohydrate-active enzymes involved in the secondary cell wall biogenesis in hybrid aspen
复制标题
DOI：
10.1104/pp.104.055087
复制
发表时间：
2005-03-01
期刊：
影响因子：
7.4
通讯作者：
Teeri, TT
中科院分区：
文献类型：
--
作者：
Aspeborg, H;Schrader, J;Teeri, TT
木材的形成是一个具有重要经济利益的基本生物过程。虽然目前对木质素的生物合成已经有了较好的了解，但导致木纤维中关键结构碳水化合物合成的途径仍然不清楚。我们利用功能基因组学方法鉴定了杂交白杨木质部发育过程中参与碳水化合物生物合成和重塑的酶。将含有不同组织特异性文库cDNA克隆的微阵列与发育木质部冷冻切片制备的窄组织切片探针杂交。利用为碳水化合物活性酶开发的灵敏工具进行生物信息学分析，鉴定出25种木质特异性糖基转移酶，属于碳水化合物活性酶家族GT2、GT8、GT14、GT31、GT43、GT47和GT61，以及9种糖苷酶（或转糖苷酶），属于碳水化合物活性酶家族GH9、GH10、GH16、GH17、GH19、GH28、GH35和GH51。虽然在次级壁特异性基因中没有发现编码多糖裂解酶或碳水化合物酯酶的基因，但鉴定了一种推定的o -乙酰转移酶。这些木材特异性酶基因为未来开发具有不同应用性能的工程纤维提供了宝贵的资源。
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the key structural carbohydrates in wood fibers remain obscure. We have used a functional genomics approach to identify enzymes involved in carbohydrate biosynthesis and remodeling during xylem development in the hybrid aspen Populus tremula x tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were hybridized with probes obtained from narrow tissue sections prepared by cryosectioning of the developing xylem. Bioinformatic analyses using the sensitive tools developed for carbohydrate-active enzymes allowed the identification of 25 xylem-specific glycosyltransferases belonging to the Carbohydrate-Active EnZYme families GT2, GT8, GT14, GT31, GT43, GT47, and GT61 and nine glycosidases (or transglycosidases) belonging to the Carbohydrate-Active EnZYme families GH9, GH10, GH16, GH17, GH19, GH28, GH35, and GH51. While no genes encoding either polysaccharide lyases or carbohydrate esterases were found among the secondary wall-specific genes, one putative O-acetyltransferase was identified. These wood-specific enzyme genes constitute a valuable resource for future development of engineered fibers with improved performance in different applications.