Actively controlled release of Dexamethasone from neural microelectrodes in a chronic in vivo study
Actively controlled release of Dexamethasone from neural microelectrodes in a chronic in vivo study
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DOI：
10.1016/j.biomaterials.2017.03.019
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发表时间：
2017-06-01
期刊：
影响因子：
14
通讯作者：
Asplund, M.
中科院分区：
文献类型：
--
作者：
Buehler, C.;Kleber, C.;Asplund, M.
长时间内与体内神经元的稳定连接对于未来先进神经电子应用的成功至关重要。植入物不可避免的异物反应挑战了植入物的稳定性，积极干预策略是局部治疗炎症的理想选择。在这里，我们研究了大鼠海马中柔性神经微电极控释消炎药地塞米松是否对植入12周的探针组织整合有影响。药物储存在导电聚合物涂层（PEDOT/Dex）中，选择性沉积在神经探针的电极上，并在全醒动物的三种电极配置下通过循环伏安法信号每周释放一次。在整个慢性研究过程中，去功能化探针提供了稳定的记录和阻抗特性。植入12周后的组织学评估显示，所有柔性探针周围的炎症程度总体较低，而与对照组相比，暴露于活性药物释放方案的电极确实有更靠近电极位置的神经元。柔性探针技术与抗炎涂层的结合为实现长期稳定的神经界面提供了一种有前途的方法。(C) 2017年作者。Elsevier Ltd.出版。
Stable interconnection to neurons in vivo over long time-periods is critical for the success of future advanced neuroelectronic applications. The inevitable foreign body reaction towards implanted materials challenges the stability and an active intervention strategy would be desirable to treat inflammation locally. Here, we investigate whether controlled release of the anti-inflammatory drug Dexamethasone from flexible neural microelectrodes in the rat hippocampus has an impact on probe-tissue integration over 12 weeks of implantation. The drug was stored in a conducting polymer coating (PEDOT/Dex), selectively deposited on the electrode sites of neural probes, and released on weekly basis by applying a cyclic voltammetry signal in three electrode configuration in fully awake animals. Dex-functionalized probes provided stable recordings and impedance characteristics over the entire chronic study. Histological evaluation after 12 weeks of implantation revealed an overall low degree of inflammation around all flexible probes whereas electrodes exposed to active drug release protocols did have neurons closer to the electrode sites compared to controls. The combination of flexible probe technology with anti-inflammatory coatings accordingly offers a promising approach for enabling long-term stable neural interfaces. (C) 2017 The Authors. Published by Elsevier Ltd.