Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS
Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS
复制标题
DOI：
10.1007/978-1-61779-319-6_23
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Sze, Siu Kwan
中科院分区：
文献类型：
--
作者：
Hao, Piliang;Zhang, Huoming;Sze, Siu Kwan
In shotgun proteomics, peptide fractionation is essential for in-depth characterization of proteomes and the mapping of protein posttranslational modifications. Recently, a mix-mode chromatography [i.e., electrostatic repulsion hydrophilic interaction chromatography (ERLIC)] has been developed and found to be a versatile method in proteome characterization. Here, we use ERLIC to characterize the glycoproteome and phosphoproteome simultaneously. We also demonstrate that the ERLIC can be an alternative to the commonly used strong cation exchange chromatography for higher recovery of proteins during whole proteome analysis. These protocols can be easily adopted in most proteomics laboratories.
