Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil
Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil
复制标题
中国土壤分枝杆菌噬菌体 SWU1 的基因组和蛋白质组学特征
DOI：
10.1016/j.gene.2015.02.053
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发表时间：
2015-04-25
期刊：
影响因子：
3.5
通讯作者：
Xie, Jianping
中科院分区：
文献类型：
--
作者：
Fan, Xiangyu;Yan, Jianlong;Xie, Jianping
分枝杆菌噬菌体SWU 1是从四川省土壤中分离到的一种新的噬菌体，以耻垢分枝杆菌mc（2）155为宿主。对噬菌斑、噬菌体形态和一步生长曲线进行了表征。通过鸟枪测序确定噬菌体SWU 1的全基因组序列。确定了SWU 1的末端。采用NanoLC-ES 1-MS/MS分析SWU 1基因组结构蛋白，鉴定出7个结构蛋白。SWU 1斑块的遗传基础进行了探讨，使用比较基因组学。在两种病原体Segniliparus rugosus ATCC BAA-974和罗得西亚分枝杆菌JS 60中鉴定出与SWU 1同源的propylene。Segniliparus属是棒状杆菌目（Corynebacteriales）的成员。据我们所知，这是分枝杆菌原噬菌体在不同属中的首次报道。(C)2015年由Elsevier B. V.出版
Mycobacteriophage SWU1 is a newly isolated phage from soil sample collected in Sichuan province, China using Mycobacterium smegmatis mc(2)155 as host. Plaque, phage morphology and one-step growth curve were characterized. The complete genomic sequence of phage SWU1 was determined by shotgun sequencing. The ends of SWU1 were determined. Structural proteins of SWU1 were analyzed by NanoLC-ES1-MS/MS. Seven ORFs were identified as structural protein encoded by SWU1 genome. The genetic basis underlying the SWU1 plaque was explored using comparative genomics. Prophages homologous to SWU1 were identified in two pathogens, Segniliparus rugosus ATCC BAA-974 and Mycobacterium rhodesiae JS60. Genus Segniliparus is a member of the order Corynebacteriales. To our knowledge, this is the first report of Mycobacterium prophages in different genera. (C) 2015 Published by Elsevier B.V.