ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN HISTIOCYTIC LYMPHOMA CELL LINE (U-937)
ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN HISTIOCYTIC LYMPHOMA CELL LINE (U-937)
复制标题
DOI：
10.1002/ijc.2910170504
复制
发表时间：
1976-01-01
影响因子：
6.4
通讯作者：
NILSSON, K
中科院分区：
文献类型：
--
作者：
SUNDSTROM, C;NILSSON, K
一株具有特殊特性的人造血细胞系(U-937)是从一例全身性组织细胞淋巴瘤患者中分离出来的。该细胞系的形态与衍生该细胞系的胸腔积液中的肿瘤细胞相同。由于EB病毒携带与肿瘤群体无关的二倍体淋巴母细胞系通常是从非Burkitt淋巴瘤外植体在体外建立的，因此对U-937进行了几项参数的研究：体外形态、生长特性、细胞化学、表面受体模式、Ig产生、溶菌酶产生、β-2-微球蛋白产生、EBV基因组存在和核型。在所有这些方面，U-937都不同于原始的淋巴母细胞系。细胞产生溶菌酶的能力和较强的酯酶活性(NaF抑制的萘酚AS-D醋酸酯酶)表明该细胞系为组织细胞起源。因此，U-937是一种肿瘤性的组织细胞系。
A human hematopoietic cell line (U‐937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. The morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. Since Epstein‐Barr virus (EBV) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non‐Burkitt lymphoma explants, several parameters were studied to discriminate the U‐937 from such lines: morphology in vitro, growth characteristics, cytochemistry, surface receptor pattern, Ig production, lysozyme production, β2‐micro‐globulin production, presence of EBV genome and karyotype. In all these respects U‐937 differed from prototype lymphoblastoid cell lines. The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity (naphtol AS‐D acetate esterase inhibited by NaF) of the cells. It is therefore concluded that the U‐937 is a neoplastic, histiocytic cell line.