Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults
Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults
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DOI：
10.1186/s12903-019-0967-2
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发表时间：
2019-12-02
期刊：
影响因子：
2.9
通讯作者：
Watanabe, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Iwai, Komei;Watanabe, Isao;Watanabe, Yoshiyuki
背景幽门螺杆菌（Helicobacter pylori，H.幽门螺杆菌）在胃中定植，被认为是胃癌的病原体。口腔是胃的传播途径，但确切的定植部位尚未明确。我们的研究调查了H。幽门螺杆菌感染和口腔样品中的存在。方法对192例口腔门诊患者的牙髓、龈上菌斑和唾液进行检测。螺杆pylori ureA基因通过巢式PCR鉴定。尿抗H。采用幽门螺杆菌抗体试验检测感染。结果25例受检者抗体阳性。牙髓PCR分析显示23例患者具有ureA基因。21例受试者牙髓中抗体和基因均呈阳性。PCR检测显示2例受试者牙菌斑阳性，但唾液阴性。H. pylori的检测结果表明存在严重龋齿的临床症状。结论H. pylori感染者表达H.幽门螺旋杆菌的样本。口腔内感染的主要储库被确定为牙髓。此外，H.幽门螺杆菌很可能从龋齿传播到根管。
Background Helicobacter pylori (H. pylori) colonize the stomach and are considered an etiological agent of gastric cancer. The oral cavity is a transmission route to the stomach, but the exact site of colonization has not yet been explicated. Our study investigated the association between H. pylori infection and presence in oral samples. Methods Dental pulp, supragingival plaque, and saliva from 192 patients visiting the Dentistry's outpatient clinic were collected for testing. The H. pylori ureA gene was identified via Nested PCR. Urine anti-H. pylori antibody test was utilized to detect infection. Results Twenty-five subjects were found to be antibody-positive. PCR analysis of dental pulp revealed that 23 subjects possessed the ureA gene. Twenty-one subjects were positive for both antibodies and genes in dental pulp. PCR testing revealed that 2 subjects were positive in dental plaque but negative for saliva. The subjects positive for H. pylori in dental pulp expressed clinical signs of severe dental caries. Conclusions H. pylori infected subjects expressed H. pylori in samples from the oral cavity. The main reservoir for infection within the oral cavity was determined to be dental pulp. Moreover, H. pylori are likely transmitted from dental caries to the root canal.