ANTIGENIC VARIATION AMONG MURINE CORONAVIRUSES - EVIDENCE FOR POLYMORPHISM ON THE PEPLOMER GLYCOPROTEIN, E2
ANTIGENIC VARIATION AMONG MURINE CORONAVIRUSES - EVIDENCE FOR POLYMORPHISM ON THE PEPLOMER GLYCOPROTEIN, E2
复制标题
DOI：
10.1016/0168-1702(85)90028-0
复制
发表时间：
1985-01-01
期刊：
影响因子：
5
通讯作者：
BUCHMEIER, MJ
中科院分区：
文献类型：
--
作者：
TALBOT, PJ;BUCHMEIER, MJ
用28种抗小鼠肝炎病毒-4株JHM （MHV-4）结构蛋白的单克隆抗体（MAb）进行3次抗原结合试验，以确定6株小鼠冠状病毒之间抗原同源性的程度。所研究的抗原决定因子在所有菌株的E1糖蛋白和核衣壳(N)蛋白上高度保守。E2糖蛋白之间存在抗原多态性。在先前描述的中和抗体针对的3个抗原决定因子中，只有1个被称为A(E2)，在所有菌株上都是保守的。抗原位点B（E2）仅在强嗜神经型MHV-4上发现，而C（E2）在强致病性MHV-4和MHV-3（嗜肝型）毒株上存在，而在弱致病性MHV-A59、MHV-1和MHV-S毒株上不存在。四种非中和抗体针对至少1种不同的地形抗原决定因子，先前被指定为D(E2)，给出了与2个不同位点一致的结合模式。其中，1存在于所有MHV毒株中，另1存在于除MHV- s外的所有毒株中。这些非中和抗原位点分别被重新命名为E（E2）和D（E2）。
A panel of 28 monoclonal antibodies (MAb) against the structural proteins of murine hepatitis virus-4, strain JHM (MHV-4) was used in 3 antigen binding assays to determine the extent of antigenic homology among 6 strains of murine coronaviruses. The antigenic determinants studied were highly conserved on the E1 glycoproteins and nucleocapsid (N) proteins of all strains tested. In contrast, antigenic polymorphism was observed among the E2 glycoproteins. Of 3 previously described antigenic determinants against which neutralizing antibodies are directed, only 1, termed A(E2), was conserved on all strains. Antigenic site B(E2) was found only on the strongly neurotropic MHV-4 and site C(E2) was present on the virulent MHV-4 and MHV-3 (hepatotropic) strains, but absent on the weakly pathogenic MHV-A59, MHV-1 and MHV-S strains. Four non-neutralizing antibodies against at least 1 topographically distinct antigenic determinant, which was previously designated D(E2), gave binding patterns consistent with 2 distinct sites. Of these, 1 was present on all MHV strains tested and the other was present on all strains except MHV-S. These non-neutralizing antigenic sites were redesignated E(E2) and D(E2) respectively.