Encapsulation of Amoxicillin within Laponite-Doped Poly(lactic-co-glycolic acid) Nanofibers: Preparation, Characterization, and Antibacterial Activity
Encapsulation of Amoxicillin within Laponite-Doped Poly(lactic-co-glycolic acid) Nanofibers: Preparation, Characterization, and Antibacterial Activity
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DOI：
10.1021/am302130b
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发表时间：
2012-11-01
影响因子：
9.5
通讯作者：
Shi, Xiangyang
中科院分区：
文献类型：
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作者：
Wang, Shige;Zheng, Fuyin;Shi, Xiangyang
我们报告了一种简便的方法来封装阿莫西林（AMX）内的锂皂石（AMX）掺杂的聚（乳酸-羟基乙酸）（PLGA）纳米纤维的生物医学应用。在这项研究中，一种合成粘土材料，纳米盘，首先被用来封装AMX。然后，通过静电纺丝将具有9.76 +/-0.57%的优化AMX负载效率的负载AMX的PLGA纳米盘并入PLGA纳米纤维中以形成混合PLGA/AMX/AMX纳米纤维。AMX在PLGA纳米盘内的负载和PLGA/AMX在PLGA纳米纤维内的负载通过不同的技术表征。在体外药物释放曲线，抗菌活性，和所形成的混合PLGA/AMX/纳米纤维的细胞相容性进行了研究。我们表明，AMX内的负载的nanodisks不会导致的nanodisks的形态和晶体结构的变化和掺入的nanodisks/AMX的PLGA纳米纤维的形态没有显着改变。重要的是，在LAP掺杂的PLGA纳米纤维内装载AMX使得AMX能够持续释放，比在单个载体的纳米盘或PLGA纳米纤维内慢得多。进一步的抗微生物活性和细胞相容性测定表明，AMX对金黄色葡萄球菌的模型细菌的生长抑制的抗微生物活性在加载到杂化纳米纤维中后没有受到损害，并且PLGA/AMX/AMX纳米纤维显示出良好的细胞相容性，类似于纯PLGA纳米纤维。有机/无机杂化纳米纤维给药系统具有良好的缓释特性和药物活性，在组织工程和药物科学中有着广泛的应用前景。
We report a facile approach to encapsulating amoxicillin (AMX) within laponite (LAP)-doped poly(lactic-co-glycolic acid) (PLGA) nanofibers for biomedical applications. In this study, a synthetic clay material, LAP nanodisks, was first used to encapsulate AMX. Then, the AMX-loaded LAP nanodisks with an optimized AMX loading efficiency of 9.76 +/- 0.57% were incorporated within PLGA nanofibers through electrospinning to form hybrid PLGA/LAP/AMX nanofibers. The loading of AMX within LAP nanodisks and the loading of LAP/AMX within PLGA nanofibers were characterized via different techniques. In vitro drug release profile, antimicrobial activity, and cytocompatibility of the formed hybrid PLGA/LAP/AMX nanofibers were also investigated. We show that the loading of AMX within LAP nanodisks does not lead to the change of LAP morphology and crystalline structure and the incorporation of LAP/AMX nanodisks does not significantly change the morphology of the PLGA nanofibers. Importantly, the loading of AMX within LAP-doped PLGA nanofibers enables a sustained release of AMX, much slower than that within a single carrier of LAP nanodisks or PLGA nanofibers. Further antimicrobial activity and cytocompatibility assays demonstrate that the antimicrobial activity of AMX toward the growth inhibition of a model bacterium of Staphylococcus aureus is not compromised after being loaded into the hybrid nanofibers, and the PLGA/LAP/AMX nanofibers display good cytocompatibility, similar to pure PLGA nanofibers. With the sustained release profile and the reserved drug activity, the organic/inorganic hybrid nanofiber-based drug delivery system may find various applications in tissue engineering and pharmaceutical science.