Uptake and Intracellular Fate of Fluorescent-Magnetic Glyco-nanoparticles
Uptake and Intracellular Fate of Fluorescent-Magnetic Glyco-nanoparticles
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DOI：
10.1002/adhm.201200051
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发表时间：
2012-05-01
影响因子：
10
通讯作者：
Penades, Soledad
中科院分区：
文献类型：
--
作者：
Gallo, Juan;Genicio, Nuria;Penades, Soledad
基于氧化铁的纳米颗粒正在寻找他们的方式作为纳米技术应用于医学的主要演员。磁铁矿纳米颗粒目前正被用于临床检测肝肿瘤的基础上，他们在肝脏中的非特异性积累。越来越多的工作正在发表在诊断和治疗的磁性纳米粒子的潜在应用。但是，磁性纳米颗粒与人类细胞在亚细胞水平上的相互作用现在才开始研究，这一领域还需要更多的基础研究。这项工作研究了碳水化合物功能化的金包被的磁铁矿纳米粒子和C33肿瘤人类细胞之间的相互作用，作为这些纳米粒子在体内应用的第一步。这种磁性材料的吸收遵循与其他纳米颗粒所描述的类似的趋势。这些纳米颗粒一旦内化的细胞内命运已经被揭开，并且用于分析共定位数据的自动方法也已经被测试并与更经典的方法进行比较。这些结果可以帮助优化磁性纳米粒子的设计，这取决于它们的最终应用。
Iron oxide based nanoparticles are finding their way as leading actors in nanotechnology applications to medicine. Magnetite nanoparticles are currently being used in clinics for the detection of hepatic tumors based on their unspecific accumulation in liver. More and more works are being published on potential applications of magnetic nanoparticles in diagnostics and therapy. But the interaction between magnetic nanoparticles and human cells at the subcellular level is only now beginning to be studied and more basic research is needed in this field. This work studies the interaction between carbohydrate functionalized gold-coated magnetite nanoparticles and C33 tumoural human cells as the first step towards the in vivo application of these nanoparticles. The uptake of this magnetic material follows a similar trend to that described for other nanoparticles. The intracellular fate of these nanoparticles once internalized has been unveiled, and an automatic methodology for the analysis of co-localization data has also been tested and compared to a more classic approach. These results can help in the optimization of the design of magnetic nanoparticles depending on their final application.