Proteomic study of sera from patients with bladder cancer: usefulness of S100A8 and S100A9 proteins.
Proteomic study of sera from patients with bladder cancer: usefulness of S100A8 and S100A9 proteins.
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发表时间：
2010-07
影响因子：
2.5
通讯作者：
S. Minami;Yuichi Sato;Toshihide Matsumoto;Taihei Kageyama;Y. Kawashima;K. Yoshio;Junnichi Ishii;Kazumasa Matsumoto;R. Nagashio;I. Okayasu
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文献类型：
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作者：
S. Minami;Yuichi Sato;Toshihide Matsumoto;Taihei Kageyama;Y. Kawashima;K. Yoshio;Junnichi Ishii;Kazumasa Matsumoto;R. Nagashio;I. Okayasu
为了有效获得肿瘤特异性标志物，通过二维凝胶电泳对来自膀胱癌患者的患者匹配的术前和术后血清使用反相高效液相色谱获得的分级蛋白进行比较。免疫组织化学证实了所鉴定蛋白质的有用性。 S100A8和S100A9被鉴定为肿瘤相关蛋白。 S100A8蛋白免疫反应性表达增加与肿瘤膀胱壁肌肉侵袭和癌症特异性生存相关（p<0.05），S100A9蛋白免疫反应性表达增加与肿瘤分级相关（p<0.05）。此外，两种蛋白表达的增加与中位随访 32.9 个月的无复发生存期相关（均 p<0.05）。在多变量分析中，S100A8 的表达是复发的显着预测因子 (p<0.05)。这些发现可能有助于识别具有生物侵袭性的肿瘤，从而有助于识别可能受益于更强化的辅助治疗的患者。
To effectively obtain tumour-specific markers, fractionated proteins obtained using reversed-phase high-performance liquid chromatography for patient-matched pre- and postoperative sera from bladder cancer patients were compared by two-dimensional gel electrophoresis. The usefulness of the identified proteins was confirmed immunohistochemically. S100A8 and S100A9 were identified as tumour-associated proteins. The increased immunoreactive expression of S100A8 protein was associated with bladder wall muscle invasion of the tumour and cancer-specific survival (p<0.05), and the increased immunoreactive expression of S100A9 protein was associated with the tumour grade (p<0.05). In addition, increased expressions of both proteins was associated with recurrence-free survival at a median follow-up of 32.9 months (both p<0.05). On multivariate analysis, the expression of S100A8 was a significant predictor of recurrence (p<0.05). These findings may help to identify biologically aggressive tumors and, thus, patients who might benefit from more intensive adjuvant therapy.