Extensive cross-contamination of specimens with Mycobacterium tuberculosis in a reference laboratory.
Extensive cross-contamination of specimens with Mycobacterium tuberculosis in a reference laboratory.
复制标题
参考实验室中标本与结核分枝杆菌发生广泛交叉污染。
DOI：
10.1128/jcm.37.4.916-919.1999
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发表时间：
1999
影响因子：
9.4
通讯作者：
Musser,JM
中科院分区：
文献类型：
--
作者：
deCRamos,M;Soini,H;Roscanni,GC;Jaques,M;Villares,MC;Musser,JM
A striking increase in the numbers of cultures positive forMycobacterium tuberculosiswas noticed in a mycobacterial reference laboratory in Campinas, Sao Paulo State, Brazil, in May 1995. A contaminated bronchoscope was the suspected cause of the increase. All 91M. tuberculosisisolates grown from samples from patients between 8 May and 18 July 1995 were characterized by spoligotyping and IS6110fingerprinting. Sixty-one of the 91 isolates had identical spoligotype patterns, and the pattern was arbitrarily designated S36. The 61 specimens containing these isolates had been processed and cultured in a 21-day period ending on 1 June 1995, but only 1 sample was smear positive for acid-fast bacilli. The patient from whom this sample was obtained was considered to be the index case patient and had a 4+ smear-positive lymph node aspirate that had been sent to the laboratory on 10 May. Virtually all organisms with spoligotype S36 had the same IS6110fingerprint pattern. Extensive review of the patients’ charts and investigation of laboratory procedures revealed that cross-contamination of specimens had occurred. Because the same strain was grown from all types of specimens, the bronchoscope was ruled out as the outbreak source. The most likely source of contamination was a multiple-use reagent used for specimen processing. The organism was cultured from two of the solutions 3 weeks after mock contamination. This investigation strongly supports the idea thatM. tuberculosisgrown from smear-negative specimens should be analyzed by rapid and reliable strain differentiation techniques, such as spoligotyping, to help rule out laboratory contamination.
