Characterisation of gastric ghrelin cells in man and other mammals: studies in adult and fetal tissues
Characterisation of gastric ghrelin cells in man and other mammals: studies in adult and fetal tissues
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DOI：
10.1007/s00418-002-0415-1
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发表时间：
2002-06-01
影响因子：
2.3
通讯作者：
Solcia, E
中科院分区：
文献类型：
--
作者：
Rindi, G;Necchi, V;Solcia, E
Ghrelin是一种新的胃肽，参与食物摄取控制和生长激素释放。我们的目的是评估其细胞定位在成人和胎儿的生活，并澄清目前种间不一致的胃内分泌细胞类型。针对生长素释放肽的氨基酸13-28产生的特异性血清在胎儿和成人胃粘膜上进行了测试，并与生长素释放肽原位杂交进行了比较。对正常人、大鼠和犬成年胃进行免疫金电镜检查。从妊娠第10周开始，在发育中的肠道、胰腺和肺以及成年人、大鼠和狗的胃粘膜中检测到Ghrelin细胞。通过免疫金电子显微镜，胃ghrelin细胞表现出独特的形态和激素反应，组胺肠嗜铬样，生长抑素D，胰高血糖素A或5-羟色胺肠嗜铬细胞。Ghrelin细胞的特征为圆形、致密、电子致密的分泌颗粒，人为P/D-1型（平均直径147 ± 30 nm），大鼠为A型（183 ± 37 nm），狗为X型（273 ± 49 nm）。它的结论是，ghrelin是由明确定义的细胞类型，这在过去已被标记不同的各种哺乳动物，主要是因为它们的分泌颗粒的大小不同。在人类，生长素释放肽细胞在胎儿早期发育。
Ghrelin is a new gastric peptide involved in food intake control and growth hormone release. We aimed to assess its cell localisation in man during adult and fetal life and to clarify present interspecies inconsistencies of gastric endocrine cell types. A specific serum generated against amino acids 13-28 of ghrelin was tested on fetal and adult gastric mucosa and compared with ghrelin in situ hybridisation. Immunogold electron microscopy was performed on normal human, rat and dog adult stomach. Ghrelin cells were detected in developing gut, pancreas and lung from gestational week 10 and in adult human, rat and dog gastric mucosa. By immunogold electron microscopy, gastric ghrelin cells showed distinctive morphology and hormone reactivity in respect to histamine enterochromaffin-like, somatostatin D, glucagon A or serotonin enterochromaffin cells. Ghrelin cells were characterised by round, compact, electrondense secretory granules of P/D-1 type in man (mean diameter 147 +/- 30 nm), A-like type in the rat (183 +/- 37 nm) and X type in the dog (273 +/- 49 nm). It is concluded that, ghrelin is produced by well-defined cell types, which in the past had been labelled differently in various mammals mostly because of the different size of their secretory granule. In man ghrelin cells develop during early fetal life.