Isolation and laboratory maintenance of Treponema pallidum.
Isolation and laboratory maintenance of Treponema pallidum.
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DOI：
10.1002/9780471729259.mc12a01s7
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发表时间：
2007-11-01
影响因子：
--
通讯作者：
Marra, Christina M
中科院分区：
文献类型：
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作者：
Lukehart, Sheila A;Marra, Christina M
引起梅毒、雅司病和bejel的螺旋体细菌不能在体外培养。本单元描述了从临床标本中分离梅毒螺旋体亚种和其他致病性梅毒螺旋体的方法，这些分离株在家兔中的繁殖，梅毒螺旋体克隆种群的分离，以及这些梅毒螺旋体冷冻储存的维持技术。
The spirochetal bacteria that cause syphilis, yaws, and bejel cannot be cultivated in vitro. This unit describes methods for the isolation of subspecies of Treponema pallidum and other pathogenic treponemes from clinical specimens, the propagation of these isolates in rabbits, isolation of clonal populations of T. pallidum, and techniques for maintenance of frozen stocks of these treponemes.