Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method
Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method
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DOI：
10.1152/ajpcell.00298.2003
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发表时间：
2004-02-01
影响因子：
5.5
通讯作者：
Verkman, AS
中科院分区：
文献类型：
--
作者：
Solenov, E;Watanabe, H;Verkman, AS
应用钙黄绿素荧光猝灭法测定野生型和水通道蛋白-4（AQP-4）缺陷小鼠脑星形胶质细胞高分化原代培养物的渗透水通透性。在盖玻片上生长的细胞用钙黄绿素加载，用于测量渗透激发后的体积变化。低渗休克产生两倍的细胞肿胀，导致钙黄绿素荧光可逆性增加12%，这与细胞溶质钙黄绿素浓度无关，其水平远低于发生钙黄绿素自猝灭的水平。钙黄绿素荧光被猝灭，
A calcein fluorescence quenching method was applied to measure osmotic water permeability in highly differentiated primary cultures of brain astrocytes from wild-type and aquaporin-4 (AQP-4)-deficient mice. Cells grown on coverglasses were loaded with calcein for measurement of volume changes after osmotic challenge. Hypotonic shock producing twofold cell swelling resulted in a reversible similar to12% increase in calcein fluorescence, which was independent of cytosolic calcein concentration at levels well below where calcein self-quenching occurs. Calcein fluorescence was quenched in