CHARACTERIZATION OF ESCHERICHIA-COLI BACTERIAL VIRUSES IN COMMERCIAL SERA
CHARACTERIZATION OF ESCHERICHIA-COLI BACTERIAL VIRUSES IN COMMERCIAL SERA
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DOI：
10.1007/bf02615323
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
MERRIL, CR
中科院分区：
文献类型：
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作者：
GEIER, MR;ATTALLAH, AFM;MERRIL, CR
方法和步骤：将市售胎牛血清接种于抗E. coliC，一株限制性酶切和修饰缺陷型菌株。观察到各种透明和浑浊斑块类型。选择具有清晰中心的四个斑块并指定为CS1、CS2、CS3和CS4。噬菌斑通过在E. coliC.从每个噬菌斑制备每ml含有超过108个噬菌斑形成单位的无菌平板原液。E.选择大肠杆菌C突变体是因为它们能够抵抗四种胎牛血清病毒之一的感染：CS 1、CS 2、CS 3和CS 4。
METHODS AND RESULTSCommercially available fetal calf serum was plated against E. coli C, a restriction-and modi-fication-deficient strain. Various clear and turbid plaque types were observed. Four plaques with clear centers were selected and designated CS1, CS2, CS3, and CS4. The plaques were purified by repeated passage on E. coli C. A sterile plate stock, containing more than 108 plaque-forming units per ml, was prepared from each plaque. E. coli C mutants were selected for their abil-ity to resist infection by one of the four fetal calf serum viruses: CS1, CS2, CS3, and CS4.