Astroglial induction of in vivo angiogenesis.
Astroglial induction of in vivo angiogenesis.
复制标题
DOI：
10.1155/np.1994.1
复制
发表时间：
1994-01
期刊：
影响因子：
--
通讯作者：
Fernández B
中科院分区：
文献类型：
--
作者：
Suárez I;Bodega G;Rubio M;García-Segura LM;Fernández B
从胚胎大鼠下丘脑中制备了纯化的星形胶质细胞。将这些细胞移植到成年大鼠的大脑皮层，存活5 d，用胶质纤维酸性蛋白（GFAP）免疫组化和电镜观察。从植入物的边缘到完整的宿主组织，观察到血管化过程的渐进渐变。再生组织中可见类似毛细血管中央管腔的空腔细胞，胞质呈金色。粒子在电子显微镜下进行免疫染色。在毛细血管样管腔中可见暗突起，在其他情况下，内皮细胞外翻与星形胶质突起接触。这些发现使我们认为，毛细血管样结构可能是由空泡星形胶质细胞发展而来的，这将允许内皮细胞迁移到它们的管腔中。星形胶质细胞与内皮细胞的相互作用一直持续到内皮细胞形态分化。对目前数据的一种可能解释是星形胶质细胞可能直接和积极地参与了毛细血管形成的调节。
A purified population of astrocytes was prepared from embryonic rat hypothalamus. These cells were transplanted into the cerebral cortex of adult rats with survival time of 5 days and studied by glial fibrillary acidic protein (GFAP) immunohistochemistry and electron microscopy. A progressive gradation from the edges of the implant to the intact host tissue was observed in relation to the vascularization process. The regenerating tissue showed cells with cavities resembling capillary central lumens and their cytoplasms revealed gold. particles when they were immunostained for electron microscopy. Dark processes were seen in capillary-like lumens and, on other occasions, evaginations of endothelial cells were in contact with astroglial processes. These findings lead us to suggest, that capillary-like structures might develop from cavitated astroglial cells, which would permit the migration of endothelial cells into their lumens. Astroglial-endothelial interactions persist until endothelial cells are morphologically differentiated. One possible interpretation of the present data is that astrocytes might participate directly and actively in the regulation of capillary formation.