Tumour necrosis factor blockade increases lymphangiogenesis in murine and human arthritic joints
Tumour necrosis factor blockade increases lymphangiogenesis in murine and human arthritic joints
复制标题
DOI：
10.1136/ard.2007.083394
复制
发表时间：
2008-11-01
影响因子：
27.4
通讯作者：
Zwerina, J.
中科院分区：
文献类型：
--
作者：
Polzer, K.;Baeten, D.;Zwerina, J.
目的：研究淋巴管在实验性关节炎小鼠以及类风湿关节炎（RA）和脊柱关节炎（SpA）患者炎症关节中的存在和调节。方法：采用免疫组织化学方法分别对人肿瘤坏死因子转基因（TNFtg）小鼠滑膜组织和RA和SpA患者滑膜活检组织中的淋巴管和血管进行评估。评估进行之前和之后的TNF封锁在所有biopsies.Results：淋巴管丰富存在于hTNFtg小鼠以及RA和SpA患者的滑膜组织。淋巴管数量与滑膜炎症程度呈正相关。英夫利西单抗治疗导致淋巴管在小鼠和人类inflammatory tissue.Conclusions的形成增加：这项研究表明，TNF封锁促进RA和SpA的炎症滑膜中的淋巴管的增殖。这一发现导致了这样的假设，即促进淋巴管生成可能在细胞和液体流出发炎组织中起重要作用。
Objective: To investigate the presence and regulation of lymphatic vessels in inflamed joints of mice with experimental arthritis as well as patients with rheumatoid arthritis ( RA) and spondyloarthritis (SpA).Methods: Lymphatic vessels and blood vessels were assessed in synovial tissue of human tumour necrosis factor transgenic (TNFtg) mice and synovial biopsies from patients with RA and SpA by immunohistochemistry for podoplanin and CD31, respectively. Assessments were performed before and after TNF blockade in all biopsies.Results: Lymphatic vessels were abundantly present in the synovial tissue of hTNFtg mice as well as patients with RA and SpA. The number of lymphatic vessels was positively related to the severity of synovial inflammation. Treatment with infliximab led to an increase in the formation of lymphatic vessels in murine and human inflammatory tissue.Conclusions: This study shows that TNF blockade promotes the proliferation of lymphatic vessels in the inflamed synovium of RA and SpA. This finding leads to the assumption that promotion of lymphangiogenesis may play an important part in efflux of cells and fluid out of the inflamed tissue.