Interactions between PAMAM dendrimers and bovine serum albumin
Interactions between PAMAM dendrimers and bovine serum albumin
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DOI：
10.1016/s1570-9639(03)00117-1
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发表时间：
2003-05-30
影响因子：
3.2
通讯作者：
Palecz, B
中科院分区：
文献类型：
--
作者：
Klajnert, B;Stanislawska, L;Palecz, B
树状大分子是一类新型的高分子材料。它们是球状的、高度分枝的、单分散的大分子。由于其独特的结构，树突状大分子有望成为新型的、有效的生物医学材料，作为寡核苷酸转染剂和药物载体。更多地了解树状大分子的生物学特性对进一步研究树状大分子在治疗中的应用至关重要。本文研究了聚酰胺胺（PAMAM）树状大分子与牛血清白蛋白（BSA）相互作用的机制。PAMAM树状大分子以乙二胺为核心，支链单元由丙烯酸甲酯和乙二胺构成。我们使用了三种具有不同表面基团（-COOH, -NH2, -OH）的PAMAM树状大分子。由于牛血清白蛋白含有两个色氨酸残基，我们能够通过测量树突状分子存在时牛血清白蛋白荧光的变化来评估树突状分子对蛋白质分子构象的影响。另外，用荧光探针1-苯胺萘-8-磺酸（ANS）进行了实验。采用差示扫描量热法（DSC）研究了树状大分子对蛋白质热稳定性的影响。我们的实验表明，牛血清白蛋白与树状大分子之间的相互作用程度强烈依赖于它们的表面基团，并且对于氨基末端的树状大分子来说是最大的。(C) 2003 Elsevier Science B.V.版权所有
Dendrimers are a new class of polymeric materials. They are globular, highly branched, monodisperse macromolecules. Due to their structure, dendrimers promise to be new, effective biomedical materials as oligonucleotide transfection agents and drug carriers. More information about biological properties of dendrimers is crucial for further investigation of dendrimers in therapeutic applications.In this study the mechanism of interactions between polyamidoamine (PAMAM) dendrimers and bovine serum albumin (BSA) was examined. PAMAM dendrimers are based on an ethylenediamine core and branched units are constructed from both methyl acrylate and ethylenediamine. We used three types of PAMAM dendrimers with different surface groups (-COOH, -NH2, -OH). As BSA contains two tryptophan residues we were able to evaluate dendrimers influence on protein molecular conformation by measuring the changes in the fluorescence of BSA in the presence of dendrimers. Additionally experiments with a fluorescent probe 1-anilinonaphthalene-8-sulfonic acid (ANS) were carried out. The differential scanning calorimetry (DSC) was chosen to investigate impact on protein thermal stability upon the dendrimers.Our experiments showed that the extent of the interactions between BSA and dendrimers strongly depends on their surface groups and is the biggest for amino-terminated dendrimers. (C) 2003 Elsevier Science B.V. All rights reserved.