Immunocytochemical Localization of Anion Exchanger-2 (AE-2) in the Dorsal Root Ganglion of Adult Rats
Immunocytochemical Localization of Anion Exchanger-2 (AE-2) in the Dorsal Root Ganglion of Adult Rats
复制标题
成年大鼠背根神经节中阴离子交换器 2 (AE-2) 的免疫细胞化学定位
DOI：
10.2220/biomedres.23.213
复制
发表时间：
2002
影响因子：
1.2
通讯作者：
Y. Ogawa
中科院分区：
文献类型：
--
作者：
S. Wakisaka;S. Honma;T. Onishi;S. Toyosawa;Y. Ogawa
近一个世纪前，Otto Warburg进行了开创性的观察，发现肿瘤代谢葡萄糖的速度比正常组织快，即使在氧气存在的情况下也是如此[1,2]。自这一观察以来，许多研究都探讨了癌细胞如何增强葡萄糖摄取以及利用这些额外葡萄糖的机制[3,4]。一些证据表明，癌细胞通过协调调节酶的表达和活性来重新编程其代谢网络，以增强合成代谢过程。影响在癌症中重要的代谢酶表达的一个过程是选择性剪接。在癌细胞中表达的选择性剪接基因中有丙酮酸激酶的胚胎亚型PKM2[5]。这一观察是由Erich Eigenbrodt首创的，现在被认为是癌症代谢调节的一个主要节点[6,7]。在下面的评论中，我们描述了PKM2活性在肿瘤中被控制以促进细胞生长的机制，这项工作源于Eigenbrodt对这种糖酵解同工酶的致瘤作用的探索。丙酮酸激酶（PK）是一种糖酵解酶，可将磷酸烯醇丙酮酸（PEP）和磷酸腺苷（ADP）转化为丙酮酸和ATP。这个反应既是一个限速步骤，也是糖酵解的最后一步。在哺乳动物中，有两个基因编码四种PK同工酶[8]。替代助剂的利用
Nearly a century ago, Otto Warburg made the seminal observation that tumors metabolize glucose more rapidly than normal tissue and do so even in the presence of ox-ygen [1,2]. Since this observation, numerous studies have addressed how cancer cells enhance glucose uptake and the mechanisms by which this additional glucose is utilized [3,4]. Several lines of evidence suggest that can-cer cells reprogram their metabolic networks through coordinated regulation of enzyme expression and activi-ty to enhance anabolic processes. One process that influ-ences expression of metabolic enzymes important in cancer is alternative splicing. Among the alternatively spliced genes expressed in cancer cells is the embryonic isoform of pyruvate kinase, PKM2 [5]. This observation was pioneered by Erich Eigenbrodt and is now consid-ered as a major node in the regulation of cancer metabo-lism [6,7]. In the following commentary, we describe the mechanisms by which the activity of PKM2 is controlled in tumors to facilitate cell growth, work that has stemmed from Eigenbrodt’s efforts exploring the onco-genic role of this glycolytic isozyme. Pyruvate kinase (PK) is a glycolytic enzyme that converts phosphoenolpyruvate (PEP) and ADP into pyruvate and ATP. This reaction is both a rate-limiting step and the final step in glycolysis [6]. In mammals, there are two genes that encode four PK isozymes [8]. Alternative pro-moter utilization of the
人结肠癌和大鼠肉瘤中丝氨酸代谢中的酶不平衡。
影响因子：
8.8
作者：
Snell, K;Natsumeda, Y;Eble, J N;Glover, J L;Weber, G
通讯作者：
Weber, G