Structure of mouse metallothionein-I gene and its mRNA
Structure of mouse metallothionein-I gene and its mRNA
复制标题
小鼠金属硫蛋白-I基因及其mRNA的结构
作者：
N. Glanville;D. Durnam;R. Palmiter
金属硫蛋白是一种富含半胱氨酸的小蛋白，能与锌、镉、铜和汞等重金属结合。最近，人们对这些蛋白的兴趣主要集中在它们在锌代谢和重金属解毒中所起的作用。我们对金属硫蛋白基因的兴趣在很大程度上是因为观察到这些蛋白可以被重金属和糖皮质激素1，3诱导。为了探索这些基因的调控，我们分离了与小鼠金属硫蛋白-I(MT-I)4相对应的cDNA和基因组克隆，并用它们来证明这两种诱导剂在体内和各种细胞系5-8中都在转录水平上起作用。我们还证明了MT-I基因在镉抗性选择过程中被扩增。为了研究基因调控的机制，初级DNA序列的知识是必要的。在这里，我们给出了小鼠MT-I基因的完整序列以及5‘侧翼区的∼300碱基，该区域可能包括启动子和调控位点。通过S1核酸酶作图确定的5‘mRNA序列与先前获得的编码区和3’非翻译区序列4相结合，以允许计算机预测MT-I mRNA最稳定的二级结构。
Metallothioneins are small cysteine-rich proteins that bind heavy metals such as zinc, cadmium, copper and mercury1,2. Recent interest in these proteins has focused on the part they play in zinc metabolism and heavy metal detoxification1. Our interest in metallothionein genes stems largely from the observations that these proteins are inducible by both heavy metals and glucocorticoid hormones1,3. To explore the regulation of these genes, we have isolated cDNA and genomic clones corresponding to mouse metallothionein-I (MT-I)4, and have used them to show that both inducers act at the transcriptional level in vivo and in a wide variety of cell lines5–8. We have also shown that the MT-I gene is amplified during selection for cadmium resistance5. To investigate the mechanisms of gene regulation, knowledge of the primary DNA sequence is necessary. Here we present the entire sequence of mouse MT-I gene along with ∼300 bases of 5′ flanking region that presumably includes promoter and regulatory sites. The 5′ mRNA sequence, defined by S1 nuclease mapping, was combined with sequences of the coding and 3′ untranslated regions obtained previously4 to allow a computer prediction of the most stable secondary structure of MT-I mRNA.
小鼠金属硫蛋白-I 基因的分离和表征。
DOI：
10.1073/pnas.77.11.6511
发表时间：
1980
影响因子：
11.1
作者：
Durnam,DM;Perrin,F;Gannon,F;Palmiter,RD
通讯作者：
Palmiter,RD
糖皮质激素对培养小鼠细胞中金属硫蛋白-I mRNA 合成的调节。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mayo,KE;Palmiter,RD
通讯作者：
Palmiter,RD
重金属对小鼠金属硫蛋白-I 基因的转录调控。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Durnam,DM;Palmiter,RD
通讯作者：
Palmiter,RD