Metabolic analysis of reepithelializing rabbit cornea using phosphorus-31 nuclear magnetic resonance spectroscopy.
Metabolic analysis of reepithelializing rabbit cornea using phosphorus-31 nuclear magnetic resonance spectroscopy.
复制标题
使用磷 31 核磁共振波谱对兔角膜上皮再生进行代谢分析。
DOI：
10.1007/bf02764295
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kenyon,KR
中科院分区：
文献类型：
--
作者：
Hayashi,K;Cheng,HM;Iwasaki,M;Xiong,H;Kenyon,KR
为了研究中央角膜和周边角膜（后者包括角膜缘区域）之间的代谢差异，解剖角膜并使用磷-31（31 P）核磁共振光谱进行检查。由于大多数31 P信号来源于上皮，角膜的31 P光谱主要代表上皮的代谢状态。周边角膜的光谱显示在整个角膜中检测到的所有磷共振;相反，中央角膜显示没有磷酸肌酸和甘油磷酸乙醇胺，只有低水平的ATP。这些结果表明，有较高的代谢活动在周边上皮，特别是在角膜缘区，比中央上皮。为了评估角膜上皮在再生过程中的代谢状态，我们还检查了通过机械刮除7 mm中央上皮组织后的角膜上皮再生。刮伤后24和48 h处死家兔。上皮再生角膜明显显示ATP、磷酸肌酸和磷酸糖随时间增加，但磷酸胆碱仍受抑制。这些发现表明，再上皮化角膜具有升高的能量产生水平，并且它可能已经达到更高的稳定状态，从而表明再生期间上皮的加速代谢。
To investigate metabolic differences between the central and peripheral cornea the latter including the limbal area, corneas were dissected and examined using phosphorus-31 (31P) nuclear magnetic resonance spectroscopy. Since most31P signals originate from the epithelium,31P spectra of the cornea primarily represent the metabolic state of the epithelium. The spectra of the peripheral cornea showed all phosphorus resonances detected in the whole cornea; in contrast, the central cornea showed no phosphocreatine and glycerophosrylethanolamine, and only low levels of ATP. These results indicate that there is a higher metabolic activity in the peripheral epithelium, especially in the limbal area, than in the central epithelium. To evaluate the metabolic state of corneal epithelium during regeneration, we also examined corneas reepithelializing after 7 mm of central epithelial tissue had been removed by mechanical scraping. Rabbits were killed 24 and 48 h after scraping. The reepithelializing corneas clearly showed an increase in ATP, phosphocreatine, and sugar phosphates with time, although phosphorylcholine remained depressed. These findings suggest that the reepithelializing cornea has an elevated level of energy production and that it may have reached a higher steady state, thereby indicating accelerated metabolism of the epithelium during regeneration.