Analysis of ploidy and sexual characteristics of natural isolates of Cryptococcus neoformans.
Analysis of ploidy and sexual characteristics of natural isolates of Cryptococcus neoformans.
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新型隐球菌天然分离株的倍性和性特征分析。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
K. Nishimura
中科院分区：
文献类型：
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作者：
K. Takeo;R. Tanaka;H. Taguchi;K. Nishimura
我们在这里报告一个广泛的倍性的担子菌酵母检查。对34株新生隐球菌变种进行了倍体分析。主要从日本患者中分离的新生儿，用碘化丙啶DNA染色后，通过定量荧光显微镜测定。包括两个试验菌株在内的菌株的性特征如下。24株是异宗配合的α型，1是异宗配合的a型，和2个自我受精，其余7个没有表现出性反应。以下菌株被发现是单倍体：异宗配合a菌株，24个异宗配合a菌株中的20个，和7个无性菌株中的3个。因此，C.正如这种生物的性反应所表明的那样，新形式的菌株大多数是单倍体。7个无性系中有4个为二倍体，2个为自交能育系。出乎意料的是，24个异宗配合的α菌株中有4个是二倍体。
We report here an extensive examination of the ploidy of a basidiomycetous yeast. The ploidy of 34 strains of Cryptococcus neoformans var. neoformans, isolated mostly from Japanese patients, was determined by means of quantitative fluorescence microscopy after DNA staining with propidium iodide. The sexual characteristics of the strains including the two tester strains were as follows. Twenty-four strains were of the heterothallic alpha type, 1 was of the heterothallic a type, and 2 were self fertile; the remaining 7 showed no sexual response. The following strains were found to be haploid: the heterothallic a strain, 20 of the 24 heterothallic alpha strains, and 3 of the 7 asexual strains. Thus, the C. neoformans strains were mostly haploid, as the sexual response of this organism suggests. Both of the self-fertile strains and four of the seven asexual strains were diploid. Unexpectedly, 4 of the 24 heterothallic alpha strains were diploid.