ANALYSIS OF CELLULAR EXPRESSION OF GANGLIOSIDES BY GENE TRANSFECTION .2. RAT 3Y1 CELLS TRANSFORMED WITH SEVERAL DNAS CONTAINING ONCOGENES (FES, FPS, RAS AND SRC) INVARIABLY EXPRESS SIALOSYLPARAGLOBOSIDE
ANALYSIS OF CELLULAR EXPRESSION OF GANGLIOSIDES BY GENE TRANSFECTION .2. RAT 3Y1 CELLS TRANSFORMED WITH SEVERAL DNAS CONTAINING ONCOGENES (FES, FPS, RAS AND SRC) INVARIABLY EXPRESS SIALOSYLPARAGLOBOSIDE
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DOI：
10.1016/0006-291x(88)90457-3
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发表时间：
1988-01-29
影响因子：
3.1
通讯作者：
NAGAI, Y
中科院分区：
文献类型：
--
作者：
NAKAISHI, H;SANAI, Y;NAGAI, Y
将几种含有癌基因ras、src、fes或fps的dna转染到大鼠3Y1细胞中，总是会诱导唾液酰副叶皂苷（SPG; IV3 . α）的新合成。NeuAc nLcOse4 Cer)，同时GM3降低。这些癌基因都是“核外”型癌基因，其产物在细胞质或细胞膜表面表达。这些结果与将“核内”型癌基因（如腺癌）转染到相同的3Y1细胞的结果形成鲜明对比，在3Y1细胞中，GD3新合成是由转染特异性引起的。
The transfection of several DNAs, containing oncogenes ras, src, fes or fps, into rat 3Y1 cells invariably induced the neosynthesis of sialosylparagloboside (SPG; IV3 .alpha. NeuAc nLcOse4 Cer), with a concomitant decrease in GM3. All these oncogenes are ''extranuclear'' type oncogenes, of which the products are expressed in the cytoplasm or on the cell surface membrane. These results are in striking contrast to those on the transfection of ''intranuclear'' type oncogenes (ex., adeno El) into the same 3Y1 cells, where GD3 neosynthesis was specifically brought about by the transfection.