Pathogenic Vibrio parahaemolyticus Increase in Intertidal-Farmed Oysters in the Mid-Atlantic Region, but Only at Low Tide
Pathogenic Vibrio parahaemolyticus Increase in Intertidal-Farmed Oysters in the Mid-Atlantic Region, but Only at Low Tide
复制标题
DOI：
10.1002/naaq.10218
复制
发表时间：
2021-12-13
影响因子：
1
通讯作者：
Bushek, David
中科院分区：
文献类型：
--
作者：
Ben-Horin, Tal;Audemard, Corinne;Bushek, David
当暴露于温暖的环境空气条件（例如潮间带暴露期间）时，弧菌属中的人类致病性海洋细菌会在贝类组织中迅速增殖。然而，目前尚不清楚潮间带养殖的贝类是否与弧菌浓度升高存在内在联系。在这里，我们对大西洋中部海岸六个实验性牡蛎养殖场的潮下和潮间带水产养殖系统中饲养的东部牡蛎（Crassostrea virginica）进行了空间匹配，并测试了潮间带水产养殖是否会提高副溶血弧菌（与海鲜相关的胃肠炎相关的主要物种）的浓度，以及携带与海鲜相关的胃肠炎相关的 tdh 和/或 trh 基因的副溶血弧菌的浓度。对人类有致病性。在各个地点，随着时间的推移，我们只发现了很少的证据表明，在低潮时暴露于环境空气中，副溶血弧菌浓度会增加。然而，tdh/trh+ 副溶血弧菌增加了约 1.5 倍。我们还发现，当高潮淹没时，潮间带和潮下线养殖的牡蛎的总副溶血弧菌浓度和tdh/trh+副溶血弧菌浓度没有差异。因此，牡蛎组织内副溶血弧菌浓度的任何变化，包括 tdh/trh+ 副溶血弧菌的浓度，都会随着潮水的到来而减弱。我们的结果共同支持许多州弧菌控制计划中现有的冷却时间限制，其中包括最大限度地减少收获的贝类在冷却或冷藏之前的保存时间，但反对更严格的措施，例如完全限制或禁止潮间带收获。
Human-pathogenic marine bacteria in the genus Vibrio proliferate rapidly in shellfish tissues when exposed to warm ambient air conditions, such as during intertidal exposure. However, it remains unclear whether intertidal-cultured shellfish are inherently associated with elevated Vibrio concentrations. Here we spatially matched eastern oysters (Crassostrea virginica) held in subtidal and intertidal aquaculture systems at six experimental oyster farms along the mid-Atlantic coast and tested whether intertidal aquaculture elevates concentrations of V. parahaemolyticus, the predominant species associated with seafood-related gastroenteritis, as well as concentrations of V. parahaemolyticus carrying the tdh and/or trh genes associated with pathogenicity in humans. Across the sites and over time, we found only slight evidence for increasing V. parahaemolyticus concentrations with exposure to ambient air at low tide; however, tdh/trh+ V. parahaemolyticus increased about 1.5-fold. We also found that total V. parahaemolyticus concentrations and concentrations of tdh/trh+ V. parahaemolyticus did not differ between intertidal- and subtidal-cultured oysters when submerged at high tides. Any changes to V. parahaemolyticus concentrations within oyster tissues, including concentrations of tdh/trh+ V. parahaemolyticus, therefore diminish with the incoming tide. Our results collectively support the existing time-to-cooling restrictions that are in place in many state Vibrio control plans, which involve minimizing the amount of time harvested shellfish can be held prior to cooling or refrigeration, but oppose more austere measures such as restricting or prohibiting intertidal harvest entirely.