Studies on seeds. I. Fixation of seeds.
Studies on seeds. I. Fixation of seeds.
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关于种子的研究。 I.种子的固定。
DOI：
10.1083/jcb.48.2.387
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发表时间：
1971-02
影响因子：
7.8
通讯作者：
Totten, C
中科院分区：
文献类型：
--
作者：
Mollenhauer, H H;Totten, C
研究了几种固定程序，以确定最适合保存种子在发育后期和萌发早期阶段。这是组织部分脱水的时期，也是电子显微镜最难固定的时期。有人发现，预固定与戊二醛的混合物，重建甲醛（即多聚甲醛），和丙烯醛，随后在OsO4或KMnO4的后固定，给出了非常可接受的图像。结果还表明，戊二醛是必要的保存细胞的形状，多聚甲醛储备蛋白质的稳定，丙烯醛快速渗透的组织。磷酸盐、二甲胂酸盐和可力丁都是可接受的缓冲液，但可力丁给出的结果最一致。
Several fixation procedures were studied to determine those most suitable for preservation of seeds during late stages of development and early stages of germination. These are the periods when the tissues are partially dehydrated and are most difficult to fix for electron microscopy. It was found that a prefixation with a mixture of glutaraldehyde, reconstituted formaldehyde (i.e. paraformaldehyde), and acrolein, followed by a postfixation in OsO4 or KMnO4, gives very acceptable images. The results also indicate that glutaraldehyde is necessary for preservation of cell shape, paraformaldehyde for stabilization of reserve proteins, and acrolein for rapid penetration of tissues. Phosphate, cacodylate, and collidine are all acceptable buffers, although collidine gives the most consistent results.