(-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury
(-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury
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DOI：
10.1007/s00702-011-0764-9
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发表时间：
2012-08-01
影响因子：
3.3
通讯作者：
Satou, Takao
中科院分区：
文献类型：
--
作者：
Itoh, Tatsuki;Imano, Motohiro;Satou, Takao
绿茶的主要成分是(-)-表没食子儿茶素没食子酸酯（EGCG），它具有很强的抗氧化特性。在这里，我们研究了EGCG对创伤性脑损伤（TBI）后受损区域周围神经干细胞（NSC）增殖的影响。在本研究中，雄性Wistar大鼠在10周龄时随意饮用正常饮用水或含有0.1% (w/v) EGCG的水，接受TBI。免疫组化结果显示，EGCG处理组脑外伤后损伤区周围巢蛋白阳性细胞数量明显高于正常水组（P < 0.05）。但EGCG处理组脑外伤后8-羟基-2′-脱氧鸟苷-、4-羟基-2-壬烯醛-、单链DNA （ssDNA）阳性细胞数量及损伤区周围过氧化水平较水组显著降低（P < 0.05）。此外，与EGCG组相比，水组几乎所有ssdna阳性细胞都与NeuN和巢蛋白染色共定位。离体研究表明，只有在脑损伤后3天，水组才能从损伤脑组织中分离出球体。然而，在EGCG组中，可以在TBI后3天和7天分离球体。EGCG组可分离出更多的球，在不含碱性成纤维细胞生长因子的培养下可分化为神经元和胶质细胞。这些结果表明，TBI前后摄入含有EGCG的水可抑制自由基诱导的NSCs降解，NSCs有可能在TBI后损伤区域周围分化为神经元和胶质细胞。
A major component of green tea is (-)-epigallocatechin gallate (EGCG), which has strong antioxidant properties. Here, we investigated the effect of EGCG on neural stem cell (NSC) proliferation around the damaged area following traumatic brain injury (TBI). In this study, male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG, ad libitum received TBI at 10 weeks of age. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the EGCG treatment group increased significantly compared with the normal water group (P < 0.05). However, the number of 8-hydroxy-2'-deoxyguanosine-, 4-hydroxy-2-nonenal-, single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly decreased in the EGCG treatment group when compared with the water group (P < 0.05). Furthermore, in contrast to the EGCG group, almost all ssDNA-positive cells in the water group co-localized with NeuN and nestin-staining. Ex vivo studies revealed that spheres could only be isolated from injured brain tissue in the water group at 3 days following TBI. However, in the EGCG group, spheres could be isolated at both 3 and 7 days following TBI. A greater number of spheres could be isolated from the EGCG group, which differentiated into neurons and glia in culture without basic fibroblast growth factor. These results indicate that consumption of water containing EGCG pre- and post-TBI inhibits free radical-induced degradation of NSCs, which have the potential to differentiate into neurons and glia around the area of damage following TBI.