A LEA model peptide protects the function of a red fluorescent protein in the dry state
A LEA model peptide protects the function of a red fluorescent protein in the dry state
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DOI：
10.1016/j.bbrep.2018.11.006
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发表时间：
2019-03-01
影响因子：
2.7
通讯作者：
Sakurai, Minoru
中科院分区：
文献类型：
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作者：
Furuki, Takao;Niwa, Tatsuya;Sakurai, Minoru
我们测试了来源于组3晚期胚胎发生丰富（G3 LEA）蛋白的短模型肽是否能够在干燥状态下维持红色荧光蛋白mKate 2的荧光活性。通过反复脱水-再水化处理，单独的mKate 2的荧光强度逐渐降低。然而，在存在莱亚模型肽的情况下，峰强度在这种强制降解处理期间几乎完全保持，这意味着即使在严重干燥条件下mKate 2的活性位点的三维结构也得到保护。为了比较，用其他添加剂如天然G3莱亚蛋白、海藻糖和BSA进行类似的实验，所有这些添加剂的保护能力都低于莱亚模型肽。
We tested whether a short model peptide derived from a group 3 late embryogenesis abundant (G3LEA) protein is able to maintain the fluorescence activity of a red fluorescent protein, mKate2, in the dry state. The fluorescence intensity of mKate2 alone decreased gradually through repeated dehydration-rehydration treatments. However, in the presence of the LEA model peptide, the peak intensity was maintained almost perfectly during such stress treatments, which implies that the three dimensional structure of the active site of mKate2 was protected even under severe desiccation conditions. For comparison, similar experiments were performed with other additives such as a native G3LEA protein, trehalose and BSA, all of whose protective abilities were lower than that of the LEA model peptide.